Gonadotropin-Releasing Hormone in Third Ventricular Cerebrospinal Fluid of the Heifer During the Estrous Cycle1
Gonadotropin-Releasing Hormone in Third Ventricular Cerebrospinal Fluid of the Heifer During the Estrous Cycle1
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发情周期小母牛第三脑室脑脊液中促性腺激素释放激素1
DOI：
10.1095/biolreprod64.2.563
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
H. Hirose
中科院分区：
文献类型：
--
作者：
K. Yoshioka;C. Suzuki;Shozo Arai;S. Iwamura;H. Hirose
摘要 研究了动情周期内卵巢完整的小母牛脑脊液（CSF）中GnRH的释放情况及其与外周血LH的相关性。使用超声检查将硅导管放入六头小母牛的第三脑室中。在黄体中期，给小母牛注射前列腺素 F2α 以诱导黄体溶解。脑脊液 GnRH（前列腺素 F2α 给药后 66.7 小时）和外周 LH（66.3 小时）同时出现激增，并且与发情期（67.0 小时）的开始一致。每头小母牛的 GnRH 浓度升高的持续时间与发情期有相当大的重叠。发情前期和黄体早期的 GnRH 和 LH 平均脉冲频率显着高于黄体中期，而这三个阶段之间 GnRH 和 LH 的平均浓度和脉冲幅度没有差异。在所有已识别的 GnRH 脉冲中，超过 80% 在发情前期和黄体早期伴随有 LH 脉冲。然而，黄体中期期间与 LH 脉冲同时发生的 GnRH 脉冲比例下降至 60%。结果清楚地表明，在小母牛的发情周期中，释放到 CSF 中的 GnRH 的动态（脉冲）和长期（激增）变化在生理上得到表达，并且小母牛中脉动 GnRH 分泌的模式取决于其发情周期。
Abstract The release profile of GnRH in cerebrospinal fluid (CSF) and its correlation with LH in peripheral blood of ovary-intact heifers during the estrous cycle were investigated. A silicon catheter was placed into the third ventricle of six heifers using ultrasonography. During the mid-luteal phase, the heifers were injected with prostaglandin F2α to induce luteolysis. Surges of CSF GnRH (66.7 h after prostaglandin F2α administration) and peripheral LH (66.3 h) occurred simultaneously and were coincident with the onset of estrus (67.0 h). Duration of elevated GnRH concentration considerably overlapped with the estrous phase in each of the heifers. Mean pulse frequencies of both GnRH and LH were significantly higher during the proestrous and early luteal phases than during the mid-luteal phase, while mean concentration and pulse amplitude of both GnRH and LH were not different between these three phases. Of all the GnRH pulses identified, more than 80% were accompanied by an LH pulse during the proestrous and early luteal phases. However, the proportion of GnRH pulses that were coincident with an LH pulse during the mid-luteal phase decreased to 60%. The results clearly demonstrate that a dynamic (pulse) and longer-term (surge) changes of GnRH release into CSF are physiologically expressed during the estrous cycle in heifers, and the pattern of pulsatile GnRH secretion in heifers depends upon their estrous cycle.
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排卵前促性腺激素激增时雌激素、孕激素和促性腺激素释放激素 (GnRH) 在控制垂体 GnRH 受体基因表达中的作用。
影响因子：
4.8
作者：
Bauer-Dantoin,AC;Weiss,J;Jameson,JL
通讯作者：
Jameson,JL
影响因子：
4.8
作者：
R. E. Rodríguez;M. Wise
通讯作者：
R. E. Rodríguez;M. Wise
去势恒河猴第三脑室脑脊液中促性腺激素释放激素脉冲：与黄体生成素脉冲的相关性。
影响因子：
4.8
作者：
VanVugt,DA;Diefenbach,WD;Alston,E;Ferin,M
通讯作者：
Ferin,M
切除卵巢、雌激素处理的恒河猴体内促性腺激素释放激素释放和血清黄体生成激素测量。
影响因子：
4.8
作者：
Levine,JE;Norman,RL;Gliessman,PM;Oyama,TT;Bangsberg,DR;Spies,HG
通讯作者：
Spies,HG
抗 GnRH 血清和酚妥拉明对 GnRH 脉冲的干扰会消除去卵巢兔子的搏动性 LH 分泌，但不会消除 FSH 分泌。
影响因子：
4.1
作者：
Pau,KY;Gliessman,PM;Oyama,T;Spies,HG
通讯作者：
Spies,HG