UT-B1 urea transporter plays a noble role as active water transporter in C6 glial cells.
UT-B1 urea transporter plays a noble role as active water transporter in C6 glial cells.
复制标题
DOI：
10.1016/j.bbrc.2006.10.097
复制
发表时间：
2006-12
影响因子：
3.1
通讯作者：
Aya Ogami;H. Miyazaki;N. Niisato;T. Sugimoto;Y. Marunaka
中科院分区：
文献类型：
--
作者：
Aya Ogami;H. Miyazaki;N. Niisato;T. Sugimoto;Y. Marunaka
由于我们的实验结果表明 UT-B1 在 C6 胶质细胞中作为对抗渗透梯度的主动水转运蛋白，我们在此首次报告主动水转运的证据。将 C6 细胞暴露于含有甘油或蔗糖的高渗溶液中，由于水根据水运动的渗透梯度流出，导致细胞收缩。另一方面，C6细胞在暴露于含有尿素的高渗溶液后就表现出细胞逆着水运动的渗透梯度而膨胀，这表明尿素加速了逆着水运动的渗透梯度的水流入；即，尿素执行主动的水运输。 UT-B 的特异性抑制剂 pCMBS 可阻止尿素引起的肿胀。在 siRNA 诱导的 UT-B1 敲低 C6 细胞中，尿素诱导的细胞肿胀被显着抑制。综上所述，这些观察结果表明 UT-B1 作为活性水转运蛋白，提供了活性水转运的新模型。
Since our experimental results suggest that UT-B1 functions as active water transporter against osmotic gradient in C6 glial cells, we report here for the first time the evidence for the active water transport. Exposure of C6 cells to a hyperosmotic solution containing glycerol or sucrose produced cell shrinkage due to water efflux according to osmotic gradient for water movement. On the other hand, C6 cells show cell swelling against osmotic gradient for water movement just after exposure to a hyperosmotic solution containing urea, indicating that water influx against osmotic gradient for water movement is accelerated by urea; i.e., urea performs active water transport. A specific inhibitor of UT-B, pCMBS, blocked the urea-induced swelling. The urea-induced cell swelling was significantly suppressed in the siRNA-induced UT-B1-knockdown C6 cells. Taken together, these observations indicate that UT-B1 acts as an active water transporter, providing a new model on active water transport.