Polymerase chain reaction primer sets for the detection of genetically diverse human sapoviruses
Polymerase chain reaction primer sets for the detection of genetically diverse human sapoviruses
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DOI：
10.1007/s00705-020-04746-9
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发表时间：
2020-07-27
影响因子：
2.7
通讯作者：
Takagi, Hirotaka
中科院分区：
文献类型：
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作者：
Oka, Tomoichiro;Yamamoto, Seiji P.;Takagi, Hirotaka
SapoVirus越来越被认为是与人类胃肠炎有关的病原体。根据主要结构蛋白编码区的序列，目前将人类皂病毒分为18种基因类型(GI.1-7、GII.1-8、GIV.1和GV.1-2)。在这项研究中，我们使用已公布的和新设计/修改的引物对11种聚合酶链式反应(PCR)方法进行了评估，结果表明，4种不同引物组合的聚合酶链式反应(PCR)方法使用合成的DNA或从人萨普病毒阳性粪便标本制备的cDNA扩增了所有被测的人萨普病毒基因型。这些检测方法可用作改进的广谱反应性筛选试验，或用作人类皂病毒的分子表征工具。
Sapoviruses are increasingly being recognized as pathogens associated with gastroenteritis in humans. Human sapoviruses are currently assigned to 18 genotypes (GI.1-7, GII.1-8, GIV.1, and GV.1-2) based on the sequence of the region encoding the major structural protein. In this study, we evaluated 11 polymerase chain reaction (PCR) assays using published and newly designed/modified primers and showed that four PCR assays with different primer combinations amplified all of the tested human sapovirus genotypes using either synthetic DNA or cDNA prepared from human sapovirus-positive fecal specimens. These assays can be used as improved broadly reactive screening tests or as tools for molecular characterization of human sapoviruses.