FISH TECHNIQUES FOR CONSTRUCTING PHYSICAL MAPS ON SCHISTOSOME CHROMOSOMES
FISH TECHNIQUES FOR CONSTRUCTING PHYSICAL MAPS ON SCHISTOSOME CHROMOSOMES
复制标题
DOI：
10.1016/0169-4758(95)80048-4
复制
发表时间：
1995-08-01
期刊：
影响因子：
--
通讯作者：
LOVERDE, PT
中科院分区：
文献类型：
--
作者：
HIRAI, H;LOVERDE, PT
为了提供有关在热带病中起重要作用的寄生虫的有用信息，世界卫生组织启动了针对许多寄生虫的基因组拖地项目。这项工作的一个目标是建立目标寄生虫基因组的物理图谱。多细胞寄生虫（蠕虫）包含不同数量的染色体，有些很大，在细胞周期中凝结。Hirohisa Hirai和Phil LaVerde详细介绍了荧光原位杂交作为一种将基因和DNA片段定位到血吸虫染色体上的手段。虽然这些技术是针对Ibr血吸虫染色体提出的，但它们适用于任何染色体在中期浓缩的系统。
in on effort to provide useful information about parasites important in tropical diseases, the WHO has initiated genome mopping projects for a number of parasites. One goal of this effort is to establish physical maps of the genomes of the targeted parasites, Multicellular parasites (helminths) contain various numbers of chromosomes, some large, that condense during the cell cycle. Here, Hirohisa Hirai and Phil LaVerde present details of fluorescence in situ hybridization as a means to localize genes and DNA fragments to schistosome chromosomes. Although the techniques presented ore Ibr schistosome chromosomes, they are applicable to any system where the chromosomes condense at metaphase.