The GUS reporter-aided analysis of the promoter activities of Arabidopsis ACC synthase genes AtACS4, AtACS5, and AtACS7 induced by hormones and stresses.
The GUS reporter-aided analysis of the promoter activities of Arabidopsis ACC synthase genes AtACS4, AtACS5, and AtACS7 induced by hormones and stresses.
复制标题
DOI：
10.1093/jxb/eri083
复制
发表时间：
2005-03
影响因子：
6.9
通讯作者：
N. Wang;M. Shih;Ning-Yan Li
中科院分区：
文献类型：
--
作者：
N. Wang;M. Shih;Ning-Yan Li
高等植物中乙烯的生物合成受到发育和环境的调节。为了研究ACC合成酶基因表达的调控，将拟南芥Acs基因的启动子AtACS4、AtACS5和AtACS7分别与GUS报告基因融合，并将重组的转基因基因导入拟南芥，获得了3组AtACS：：GUS转基因植株。对这些转基因植株的组织化学和荧光研究表明，AtACS4、AtACS和AtACS7的启动子在暗萌发的幼苗中都是活性的。在所研究的三个Atacs基因中，AtACS5在2周龄光照幼苗叶片中的启动子活性最高。在成熟叶片中，AtACS4和AtACS7基因在叶脉和乳晕中均有表达，而AtACS5在乳晕和毛状体周围的表皮细胞中表达水平较高。所有这些atacs基因的启动子活性都存在于生殖器官中。AtACS5和AtACS7在花瓣、萼片、心皮、雄蕊、茎生叶、花序茎和角果中高度表达，而AtACS4在开放花的花瓣中不表达。所有这三个atacs基因都在根组织中表达。在2周龄的光培养拟南芥中，AtACS4启动子对植物激素IAA、乙烯和ABA以及黑暗和伤害有反应；AtACS5启动子对IAA、ABA、盐、高温和伤害有反应；AtACS7启动子对GA3、乙烯和ABA以及黑暗和盐有反应。低温处理可取消黑暗诱导的AtACS7基因表达，但不能取消AtACS4基因的表达。每个atacs基因在生长发育过程中都有独特的表达谱。似乎在拟南芥的任何发育阶段或任何生长期，总有一个Atacs多基因家族的成员活跃地表达。
Ethylene biosynthesis in higher plants is regulated developmentally and environmentally. To investigate the regulation of ACC synthase gene expression, the promoters of Arabidopsis ACS genes, AtACS4, AtACS5, and AtACS7, were fused to a GUS reporter gene, and the recombinant transgenes were introduced into Arabidopsis to produce three groups of AtACS::GUS transgenic plants. Histochemic and fluorometric study of these transgenic plants revealed that promoters of AtACS4, AtACS, and AtACS7 are all active in dark-germinated seedlings. AtACS5 has the highest promoter activity in leaves of 2-week-old light-grown seedlings among the three AtACS genes studied. In the mature leaves, AtACS4 and AtACS7 genes are expressed in both veins and areoles, whereas AtACS5 is expressed at a higher level in the areoles and epidermal cells surrounding trichomes. The promoter activities of all these AtACS genes are found in the reproductive organs. AtACS5 and AtACS7 are highly expressed in petals, sepals, carpels, stamens, cauline leaves, inflorescence stems, and siliques, while AtACS4 expression is undetectable in the petals of open flowers. All three AtACS genes are expressed in root tissue. In the 2-week-old light-grown Arabidopsis, the AtACS4 promoter is responsive to the plant hormones IAA, ethylene, and ABA, and to darkness and wounding; the AtACS5 promoter to IAA, ABA, salt, high temperature, and wounding; and the AtACS7 promoter to GA3, ethylene, and ABA, and to darkness and salt. Low-temperature treatment abolishes the darkness-induced AtACS7 gene expression, but not that of AtACS4. Each AtACS gene has a unique expression profile during growth and development. It appears that at any developmental stage or any growth period of Arabidopsis, there is always a member of AtACS multigene family that is actively expressed.