Conformational properties of high molecular weight heteropolysaccharide isolated from seeds of Artemisia sphaerocephala Krasch
Conformational properties of high molecular weight heteropolysaccharide isolated from seeds of Artemisia sphaerocephala Krasch
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DOI：
10.1016/j.foodhyd.2012.12.014
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发表时间：
2013-07-01
影响因子：
10.7
通讯作者：
Yada, Rickey Y.
中科院分区：
文献类型：
--
作者：
Guo, Qingbin;Wang, Qi;Yada, Rickey Y.
研究了从白蒿 (ASK) 中提取的高分子量多糖 (60P) 的构象特性。使用静态光散射和HPSEC技术获得60P的M-w和R-g，分别为590 kDa和56 nm。动态光散射测量表明0.5 M NaOH溶剂表现出减少/消除60P聚集体的能力。 0.5 M NaOH 中的 60P 显示出强烈的浓度依赖性，但角度依赖性极小。平均结构参数rho（R-g/R-h）值为1.75，表明60P在0.5 M NaOH溶液中表现出无规卷曲构象。 HPSEC 的构象参数（源自 R-g 与 M-w 和 R-h 与 M-w）也表明 0.1 M NaNO3 溶液（HPSEC 的洗脱液）中 60P 的随机卷曲构象。随机飞行模型和蠕虫圆柱模型得到的特征比和持续长度均表明60P为半柔性结构。 Crown 版权所有 (c) 2012 由 Elsevier Ltd 出版。保留所有权利。
The conformational properties of a high molecular weight polysaccharide (60P) extracted from Artemisia sphaerocephala Krasch (ASK) were investigated. The M-w and R-g of 60P were obtained using both static light scattering and HPSEC techniques, which were 590 kDa and 56 nm, respectively. Dynamic light scattering measurement indicated that 0.5 M NaOH solvents exhibited the ability to reduce/eliminate aggregates of 60P. 60P in 0.5 M NaOH showed strong concentration dependence, but minimal angular dependence. The average structure parameter rho (R-g/R-h) value of 1.75 indicated that 60P exhibited a random coil conformation in 0.5 M NaOH solution. The conformational parameters (derived from R-g vs M-w and R-h vs M-w) from HPSEC also suggested a random coil conformation of 60P in 0.1 M NaNO3 solution (eluent for HPSEC). The characteristic ratio and the persistence length obtained from the random flight model and wormlike cylinder model both indicated a semi-flexible structure of 60P. Crown Copyright (c) 2012 Published by Elsevier Ltd. All rights reserved.