Flexible endoscope for continuous in vivo multispectral fluorescence lifetime imaging.
Flexible endoscope for continuous in vivo multispectral fluorescence lifetime imaging.
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DOI：
10.1364/ol.38.001515
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发表时间：
2013-05-01
期刊：
影响因子：
3.6
通讯作者：
Jo JA
中科院分区：
文献类型：
--
作者：
Cheng S;Rico-Jimenez JJ;Jabbour J;Malik B;Maitland KC;Wright J;Cheng YS;Jo JA
荧光寿命成像 (FLIM) 提供了一种表征生物组织生化成分的非侵入性方法。人们对多光谱 FLIM 在医学诊断中的应用越来越感兴趣。 FLIM 技术临床转化的核心是紧凑型高速内窥镜系统的开发。不幸的是，主要的多光谱 FLIM 方法存在局限性，阻碍了适合体内组织成像的内窥镜系统的开发。我们推出了一款紧凑型宽视场时间选通 FLIM 柔性内窥镜，能够同时对多达三个荧光发射带进行连续寿命成像。这种新颖的内窥镜设计将有助于评估 FLIM 在体内的应用。
Fluorescence lifetime imaging (FLIM) offers a noninvasive approach for characterizing the biochemical composition of biological tissue. There has been an increasing interest in the application of multispectral FLIM for medical diagnosis. Central to the clinical translation of FLIM technology is the development of compact and high-speed endoscopy systems. Unfortunately, the predominant multispectral FLIM approaches suffer from limitations that impede the development of endoscopy systems that are suitable for in vivo tissue imaging. We present a compact wide-field time-gated FLIM flexible endoscope capable of continuous lifetime imaging of up to three fluorescence emission bands simultaneously. This novel endoscope design will facilitate the evaluation of FLIM for in vivo applications.