Mice Cloned from Induced Pluripotent Stem Cells.
Mice Cloned from Induced Pluripotent Stem Cells.
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DOI：
10.1093/biolreprod/83.s1.711
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发表时间：
2010-11
影响因子：
3.6
通讯作者：
Shaorong Gao;Zhaohui Kou;Lan Kang;Ye Yuan;Yu Tao;Jing He;Tong Wu;Yu Zhang
中科院分区：
文献类型：
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作者：
Shaorong Gao;Zhaohui Kou;Lan Kang;Ye Yuan;Yu Tao;Jing He;Tong Wu;Yu Zhang
不同物种的分化体细胞可以通过异位表达高度富集于胚胎干细胞（ESC）中的几种转录因子的组合而重编程为诱导多能干细胞（iPSC）。大动物iPS细胞的产生为通过核移植方法生产转基因大动物提供了可能性。然而，iPSC是否可以通过体细胞核移植（SCNT）用于产生克隆动物仍然是未知的。在这里，我们展示了通过SCNT方法从诱导型iPSC成功生产可行的克隆小鼠，并且效率与使用通过正常受精衍生的ESC相似。此外，克隆小鼠具有生育能力，可以产生第二代后代。这些努力加强了未来利用iPSC产生用于制药目的的基因修饰大型动物的可能性。（海报）
Differentiated somatic cells of various species could be reprogrammed into induced pluripotent stem cells (iPSCs) by ectopically expressing a combination of several transcription factors which are highly enriched in embryonic stem cells (ESCs). The generation of iPS cells in large animals has raised the possibility of producing genetically modified large animals through nuclear transplantation approach. However, it remains unknown whether iPSCs could be used for generating cloned animals through somatic cell nuclear transfer (SCNT). Here, we show the successful production of viable cloned mice from inducible iPSCs through SCNT approach, and the efficiency is similar to using ESCs derived via normal fertilization. Furthermore, the cloned mice are fertile and can produce second-generation offspring. These efforts strengthened the possibility of utilizing iPSCs to generate gene modified large animals for pharmaceutical purposes in the future.(poster)