Expression of mmu-miR-96 in the endometrium during early pregnancy and its regulatory effects on stromal cell apoptosis via Bcl2.
Expression of mmu-miR-96 in the endometrium during early pregnancy and its regulatory effects on stromal cell apoptosis via Bcl2.
复制标题
mmu-miR-96在妊娠早期子宫内膜中的表达及其通过Bcl2对基质细胞凋亡的调节作用
DOI：
10.3892/mmr.2017.6212
复制
发表时间：
2017-04
影响因子：
3.4
通讯作者：
Pan Y
中科院分区：
文献类型：
--
作者：
Yang Y;Xie Y;Wu M;Geng Y;Li R;Xu L;Liu X;Pan Y
子宫内膜间质细胞的蜕膜化是着床和妊娠的重要特征。蜕膜化的分子机制仍不清楚，特别是关于这一过程的microRNA（miRNA/miR）调控。本研究采用逆转录-定量聚合酶链反应（RT-PCR）和原位杂交技术研究mmu-miR-96在小鼠妊娠早期子宫中的时空分布。此外，从小鼠子宫分离原代基质细胞，并用于探索mmu-miR-96在蜕膜化中的作用。结果表明，mmu-miR-96在妊娠期间在基质细胞中高度表达，并在着床部位上调。此外，mmu-miR-96在蜕膜化过程中强烈表达，这表明它可能在基质细胞的蜕膜化中起作用。鉴于mmu-miR-96参与细胞凋亡，本研究探讨mmu-miR-96对原代子宫内膜间质细胞凋亡的影响。结果表明mmu-miR-96过表达可诱导基质细胞凋亡。在关于潜在机制的进一步研究中，通过生物信息学分析筛选mmu-miR-96的靶基因，并且如使用报告基因测定所确定的，证实抗凋亡基因B细胞淋巴瘤2是mmu-miR-96的靶。总之，本研究表明mmu-miR-96参与小鼠子宫内膜间质细胞的蜕膜化，从而在妊娠中发挥关键作用。
Decidualization of endometrial stromal cells is an important feature of implantation and pregnancy. The molecular mechanism underlying decidualization remains unclear, particularly regarding the microRNA (miRNA/miR) regulation of this process. The present study revealed the temporal and spatial distribution of mmu-miR-96 in the mouse uterus during early pregnancy by reverse transcription-quantitative polymerase chain reaction and in situ hybridization. In addition, primary stromal cells were isolated from the mouse uterus and used to explore the role of mmu-miR-96 in decidualization. The results demonstrated that mmu-miR-96 was highly expressed in stromal cells during pregnancy, and was upregulated at implantation sites. In addition, mmu-miR-96 was strongly expressed during decidualization, which indicates that it may serve a role in the decidualization of stromal cells. Based on existing reports, mmu-miR-96 participates in apoptosis; therefore the present study investigated its effects on the apoptosis of primary endometrial stromal cells. The results indicated that overexpression of mmu-miR-96 may induce apoptosis of stromal cells. In further studies regarding the underlying mechanism, the target genes of mmu-miR-96 were screened by bioinformatics analysis, and it was confirmed that B-cell lymphoma 2, an anti-apoptotic gene, was the target of mmu-miR-96, as determined using a reporter gene assay. In conclusion, the present study suggested that mmu-miR-96 participates in the decidualization of endometrial stromal cells in mice, thereby serving a key role in pregnancy.