Effect of stepwise lignin removal on the enzymatic hydrolysis and cellulase adsorption
Effect of stepwise lignin removal on the enzymatic hydrolysis and cellulase adsorption
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逐步去除木质素对酶水解和纤维素酶吸附的影响
DOI：
10.1016/j.indcrop.2018.05.053
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发表时间：
2018-10
影响因子：
5.9
通讯作者：
Zhenhong Yuan
中科院分区：
文献类型：
--
作者：
Wen Wang;Xuesong Tan;Qiang Yu;Qiong Wang;Wei Qi;Xinshu Zhuang;Zhongming Wang;Zhenhong Yuan
木质素可通过非生产性酶吸附和/或空间位阻对预处理的木质纤维素的酶促水解产生负面影响。用含有不同木质素含量的甘蔗渣（SCB）样品及其保留完整原木质素的研磨木质素和酶解木质素（MEL）评估残余木质素对酶解的影响。结果表明，当木质素去除率达到64.0%以上时，对SCB酶解的促进作用不再明显，木质素化学基团的变化对吸附酶的影响不大。当木质素在预处理SCB中的保留率小于36.0%时，残余木质素不会通过酶吸附和空间位阻作用对酶解产生显著影响。预处理后的SCB样品中残留木质素对总酶吸附量的贡献仅在9.6%~ 15.5%之间，对总酶吸附量的贡献不是主要的。
Lignin can negatively impose on the enzymatic hydrolysis of pretreated lignocellulose through non-productive enzyme adsorption and/or steric hindrance. The effect of the residual lignin on enzymatic hydrolysis was evaluated with sugarcane bagasse (SCB) samples containing different lignin contents and their milled and enzymolytic lignins (MELs) retaining the intact protolignins. The results showed that the improvement of enzymatic hydrolysis of SCB would become insignificant when the lignin removal achieved above 64.0%, and the changes in chemical groups of lignin had slight impact on adsorbing enzymes. When the lignin retention in the pretreated SCB was less than 36.0%, the residual lignin would not significantly influence the enzymatic hydrolysis via enzyme adsorption and steric hindrance. The contributions of the residual lignins in pretreated SCB samples to the total enzyme adsorptions of pretreated SCB samples were only in the range of 9.6% to 15.5%, which were not predominant for the total enzyme adsorptions.
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DOI：
10.1002/chin.201550242
发表时间：
2015-12
期刊：
ChemInform
影响因子：
--
作者：
M. H. L. Silveira;A. Morais;A. Lopes;Drielly N Olekszyszen;R. Bogel-Lukasik;J. Andreaus;L. Ramos
通讯作者：
M. H. L. Silveira;A. Morais;A. Lopes;Drielly N Olekszyszen;R. Bogel-Lukasik;J. Andreaus;L. Ramos
影响因子：
4.6
作者：
Donaldson L;Vaidya A
通讯作者：
Vaidya A
影响因子：
6.3
作者：
Siqueira G;Arantes V;Saddler JN;Ferraz A;Milagres AMF
通讯作者：
Milagres AMF
DOI：
10.1002/chin.201012279
发表时间：
2009-10
期刊：
ChemInform
影响因子：
--
作者：
M. Bardet;G. Gerbaud;Mathilde Giffard;C. Doan;S. Hediger;L. L. Pape-L.
通讯作者：
M. Bardet;G. Gerbaud;Mathilde Giffard;C. Doan;S. Hediger;L. L. Pape-L.
影响因子：
3.2
作者：
Woochul Jung;D. Savithri;Ratna R. Sharma-Shivappa;P. Kolar
通讯作者：
Woochul Jung;D. Savithri;Ratna R. Sharma-Shivappa;P. Kolar