Crystal structure of ADP/AMP complex of Escherichia coli adenylate kinase
Crystal structure of ADP/AMP complex of Escherichia coli adenylate kinase
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DOI：
10.1002/prot.20699
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发表时间：
2006-02-01
影响因子：
2.9
通讯作者：
Phillips, GN
中科院分区：
文献类型：
--
作者：
Berry, MB;Bae, EY;Phillips, GN
材料和方法。如前所述表达和纯化大肠杆菌 AK。 5 使用 2.2M 硫酸铵、1% PEG 2000 单甲醚、50 mM MES pH 6.7、10 mM EDTA、0.1% 叠氮化钠以及 3 mM ADP 和 AMP，通过悬滴蒸汽扩散法将两个晶体生长到边缘尺寸为 0.3 至 0.4 mm× 0.1 mm× 0.05 mm。数据集在 R-AXIS IIc 成像板探测器上收集。发现该晶体属于空间群 P2 1 2 1 2，不对称单元中有两个分子。晶胞常数确定为a=73.5、b=84.6、c=80.3。两个数据集合并后，整体完整性达到 2.8 Å，大于 99.0%，R 合并率为 14.3%，并由 12852 个独特反射组成。
Materials and Methods.E. coli AK was expressed and purified as described previously. 5 Two crystals were grown by hanging drop vapor diffusion method to dimensions of 0.3 to 0.4 mm× 0.1 mm× 0.05 mm on edge using 2.2M ammonium sulfate, 1% PEG 2000 monomethyl ether, 50 mM MES pH 6.7, 10 mM EDTA, 0.1% sodium azide, and 3 mM ADP and AMP. Data sets were collected on an R-AXIS IIc imaging plate detector. The crystals were found to belong to the space group P2 1 2 1 2 with two molecules in the asymmetric unit. The unit cell constants were determined to be a= 73.5, b= 84.6, c= 80.3. The two data sets were merged producing an overall completeness to 2.8 Å of greater than 99.0% with an R merge of 14.3%, and consisting of 12852 unique reflections.