Reply: tissue fibrocytes are a subpopulation of macrophages.
Reply: tissue fibrocytes are a subpopulation of macrophages.
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答复：组织纤维细胞是巨噬细胞的一个亚群。
DOI：
10.1165/rcmb.2014-0218le
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发表时间：
2015
影响因子：
6.4
通讯作者：
Madala,SatishK
中科院分区：
文献类型：
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作者：
Madala,SatishK
术语“纤维细胞”是由Bucala及其同事在1994年创造的，用于描述在粘附白细胞的长期培养物中出现的细胞（1）。随后的许多报道描述了明显新颖的骨髓来源的细胞，称为纤维细胞，其在人类血液中循环并出现在组织纤维化部位。然而，循环人纤维细胞的纯化和精确的表型分析（包括显微镜）尚未报道。在他们对纤维细胞的优雅研究中，Madala及其同事证明了表达白细胞标志物CD 45的骨髓源性细胞能够浸润小鼠的纤维化肺并调节纤维化（2）。这些细胞表达髓样标记物CD 14沿着CD 44，并且能够合成胶原1，并且它们调节驻留成纤维细胞的增殖。Madala及其同事描述的这些纤维细胞与单核细胞衍生的巨噬细胞具有非常相似的特征。CD 14阳性单核细胞在骨髓中产生，在血液中循环，并被募集到组织中，在那里它们分化成巨噬细胞。组织巨噬细胞表现出很大的异质性，具有许多不同的形式和功能，在组织稳态和对损伤的反应中发挥作用;特别是，它们可以增殖，合成胶原蛋白，并调节组织纤维化（3-6）。Madala及其同事的研究结果支持了组织纤维细胞是巨噬细胞亚群的观点。如果是这样，那么将纤维细胞作为一种新的细胞类型是没有帮助的。未来的研究，结合细胞分选和形态和功能的仔细分析，将有助于解决这种不确定性。
The term “fibrocyte” was coined by Bucala and colleagues in 1994 to describe a cell that appeared in long-term cultures of adherent leukocytes (1). Many subsequent reports have described apparently novel bone marrow–derived cells, termed fibrocytes, that circulate in the bloodstream in humans and appear at sites of tissue fibrosis. However, purification and precise phenotypic analysis (including microscopy) of circulating human fibrocytes has not been reported. In their elegant study of fibrocytes in the Journal, Madala and colleagues demonstrate that bone marrow–derived cells that express the leukocyte marker CD45 are able to infiltrate fibrotic lung and modulate fibrosis in the mouse (2). These cells expressed the myeloid marker CD14 along with CD44 and were able to synthesize collagen 1, and they regulated proliferation of resident fibroblasts. These fibrocytes described by Madala and colleagues have remarkably similar characteristics to monocyte-derived macrophages. CD14-positive monocytes are produced in the bone marrow, circulate in the blood, and are recruited into tissues where they differentiate into macrophages. Tissue macrophages exhibit great heterogeneity with many different forms and functions, with roles in tissue homeostasis and responses to injury; in particular, they can proliferate, synthesize collagen, and regulate tissue fibrosis (3–6). The results of Madala and colleagues support the idea that tissue fibrocytes are a subpopulation of macrophages. If so, then reference to fibrocytes as a novel cell type is unhelpful. Future studies that incorporate cell sorting and careful analyses of morphology and function would help resolve this uncertainty.■