Grainyhead-related transcription factor is required for duct maturation in the salivary gland and the kidney of the mouse
Grainyhead-related transcription factor is required for duct maturation in the salivary gland and the kidney of the mouse
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DOI：
10.1242/dev.02658
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发表时间：
2006-12-01
期刊：
影响因子：
4.6
通讯作者：
Takada, Shinji
中科院分区：
文献类型：
--
作者：
Yamaguchi, Yoshifumi;Yonemura, Shigenobu;Takada, Shinji
导管上皮结构是许多内脏的基本特征，包括外分泌腺和肾脏。这些管道不仅起到调节液体转运的作用，还有助于维持体内平衡。例如，流体和溶质从主流体中吸收或分泌到主流体中。由于穿过外分泌腺和肾脏的管腔。这些导管功能成熟的分子机制在很大程度上仍不清楚。在这里，我们证明了与颗粒相关的转录因子CP2L1(CP2L1)是唾液腺和肾脏导管成熟所必需的。在小鼠中，CP2/1特异地表达在许多外分泌腺的发育导管中，包括唾液腺，以及肾脏的外分泌腺。在CP2/1缺陷小鼠中，直接参与导管功能成熟的基因在唾液腺和肾脏中的表达都特异性地降低，表明CP2/1是导管细胞分化所必需的。此外，这些小鼠的唾液和尿液成分异常。这些结果表明，CP2/1的表达是唾液腺和肾脏正常导管发育所必需的。
Duct epithelial structure is an essential feature of many internal organs, including exocrine glands and the kidney. The ducts not only mediate fluid transfer but also help to maintain homeostasis. For instance, fluids and solutes are resorbed from or secreted into the primary fluid. owing through the lumen of the ducts in the exocrine glands and kidneys. The molecular mechanism underlying the functional maturation of these ducts remains largely unknown. Here, we show that a grainyhead-related transcription factor, CP2-like 1 (CP2L1), is required for the maturation of the ducts of the salivary gland and kidney. In the mouse, Cp2/1 is specifically expressed in the developing ducts of a number of exocrine glands, including the salivary gland, as well as in those of the kidney. In Cp2/1-deficient mice, the expression of genes directly involved in functional maturation of the ducts was specifically reduced in both the salivary gland and kidney, indicating that Cp2/1 is required for the differentiation of duct cells. Furthermore, the composition of saliva and urine was abnormal in these mice. These results indicate that Cp2/1 expression is required for normal duct development in both the salivary gland and kidney.