Electric pulses: a flexible tool to manipulate cytosolic calcium concentrations and generate spontaneous-like calcium oscillations in mesenchymal stem cells.
Electric pulses: a flexible tool to manipulate cytosolic calcium concentrations and generate spontaneous-like calcium oscillations in mesenchymal stem cells.
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DOI：
10.1038/srep32331
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发表时间：
2016-08-26
影响因子：
4.6
通讯作者：
Mir LM
中科院分区：
文献类型：
--
作者：
de Menorval MA;Andre FM;Silve A;Dalmay C;Français O;Le Pioufle B;Mir LM
人脂肪间充质干细胞（haMSCs）是一种多能成体干细胞，在再生医学和肿瘤学领域具有重要的应用价值。它们呈现与细胞周期进展或分化相关的自发钙振荡，但这些事件之间的相关性仍不清楚。事实上，很难用化学手段模拟haMSC自发的钙振荡。脉冲电场（PEF）可以根据所施加的脉冲使血浆和/或细胞器膜透化，并且因此通过从外部介质或从内部存储募集钙来产生胞质钙峰。我们发现，使用100 μs或10 ns的PEFs可以模拟haMSCs自发的钙振荡（相同的幅度、持续时间和形状）。我们提出了一个模型，解释报告的实验情况。因此，PEFs可以是一个灵活的工具，以操纵细胞溶质钙浓度。与化学试剂相反，这种工具可以瞬时打开和关闭，对于研究钙振荡在细胞生理学中的作用和/或操纵细胞命运非常有用。
Human adipose mesenchymal stem cells (haMSCs) are multipotent adult stem cells of great interest in regenerative medicine or oncology. They present spontaneous calcium oscillations related to cell cycle progression or differentiation but the correlation between these events is still unclear. Indeed, it is difficult to mimic haMSCs spontaneous calcium oscillations with chemical means. Pulsed electric fields (PEFs) can permeabilise plasma and/or organelles membranes depending on the applied pulses and therefore generate cytosolic calcium peaks by recruiting calcium from the external medium or from internal stores. We show that it is possible to mimic haMSCs spontaneous calcium oscillations (same amplitude, duration and shape) using 100 μs PEFs or 10 ns PEFs. We propose a model that explains the experimental situations reported. PEFs can therefore be a flexible tool to manipulate cytosolic calcium concentrations. This tool, that can be switched on and off instantaneously, contrary to chemicals agents, can be very useful to investigate the role of calcium oscillations in cell physiology and/or to manipulate cell fate.