Baicalin Inhibits Ferroptosis in Intracerebral Hemorrhage.
Baicalin Inhibits Ferroptosis in Intracerebral Hemorrhage.
复制标题
黄芩苷抑制脑出血中的铁死亡。
DOI：
10.3389/fphar.2021.629379
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发表时间：
2021
影响因子：
5.6
通讯作者：
Wang Q
中科院分区：
文献类型：
--
作者：
Duan L;Zhang Y;Yang Y;Su S;Zhou L;Lo PC;Cai J;Qiao Y;Li M;Huang S;Wang H;Mo Y;Wang Q
脑出血（ICH）是卒中的一种亚型，其特征是高死亡率和致残率。迄今为止，ICH引起的脑损伤的确切病因仍不清楚。此外，目前还没有有效的治疗方法来延缓或预防疾病进展。越来越多的证据表明，铁凋亡在脑出血损伤的发病机制中起主导作用。黄芩苷是中草药黄芩的主要活性成分。据报道，它对ICH诱导的脑损伤表现出神经保护作用，并减少多种组织中的铁沉积。因此，在本研究中，我们集中在黄芩苷对脑出血引起的铁凋亡的保护机制，使用氯化血红素诱导的体外模型和IV型胶原酶诱导的体内模型。我们的研究结果表明，黄芩苷增强了氯化高铁血红素、埃拉斯汀和RSL3处理的大鼠嗜铬细胞瘤PC 12细胞的细胞活力并抑制了铁细胞凋亡。黄芩苷对原代皮层神经元（PCN）有明显的抗铁凋亡作用。黄芩苷还可通过抑制脑出血模型小鼠的铁凋亡而减轻脑出血小鼠的运动障碍和脑损伤。黄芩苷对小鼠肝、肾无明显毒性。可见，铁凋亡是脑出血的重要病理特征，黄芩苷可预防脑出血时铁凋亡的发生。因此，黄芩苷是一种潜在的治疗ICH的药物。
Intracerebral hemorrhage (ICH) is a subtype of stroke characterized by high mortality and disability rates. To date, the exact etiology of ICH-induced brain injury is still unclear. Moreover, there is no effective treatment to delay or prevent disease progression currently. Increasing evidence suggests that ferroptosis plays a dominant role in the pathogenesis of ICH injury. Baicalin is a main active ingredient of Chinese herbal medicine Scutellaria baicalensis. It has been reported to exhibit neuroprotective effects against ICH-induced brain injury as well as reduce iron deposition in multiple tissues. Therefore, in this study, we focused on the protective mechanisms of baicalin against ferroptosis caused by ICH using a hemin-induced in vitro model and a Type IV collagenase-induced in vivo model. Our results revealed that baicalin enhanced cell viability and suppressed ferroptosis in rat pheochromocytoma PC12 cells treated with hemin, erastin and RSL3. Importantly, baicalin showed anti-ferroptosis effect on primary cortical neurons (PCN). Furthermore, baicalin alleviated motor deficits and brain injury in ICH model mice through inhibiting ferroptosis. Additionally, baicalin existed no obvious toxicity towards the liver and kidney of mice. Evidently, ferroptosis is a key pathological feature of ICH and baicalin can prevent the development of ferroptosis in ICH. As such, baicalin is a potential therapeutic drug for ICH treatment.