Overexpression of Suprabasin is Associated with Proliferation and Tumorigenicity of Esophageal Squamous Cell Carcinoma.
Overexpression of Suprabasin is Associated with Proliferation and Tumorigenicity of Esophageal Squamous Cell Carcinoma.
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Suprabasin 的过度表达与食管鳞状细胞癌的增殖和致瘤性相关
DOI：
10.1038/srep21549
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发表时间：
2016-02-22
影响因子：
4.6
通讯作者：
Jiang L
中科院分区：
文献类型：
--
作者：
Zhu J;Wu G;Li Q;Gong H;Song J;Cao L;Wu S;Song L;Jiang L
Suprabasin是最近发现的一种在多种癌症中上调的癌蛋白。然而，在人食管鳞状细胞癌（ESCC）中的临床意义和生物学作用尚不清楚。在目前的研究中，我们报道了suprabasin在ESCC细胞系和组织中mRNA和蛋白水平的显著过表达，这与晚期临床分期、肿瘤淋巴结转移（TNM）分类、组织学分化、肿瘤大小和较差的生存率相关。此外，我们发现suprabasin过表达可显著诱导ESCC细胞的增殖和致瘤性，但suprabasin敲除可抑制其增殖和致瘤性。此外，我们证明了suprabasin的上调激活了Wnt/β-catenin信号通路，导致β-catenin的核定位和Cyclin D1和c-Myc的上调。综上所述，我们的研究结果表明，在ESCC的增殖和肿瘤发生过程中，上盆底蛋白酶起着重要的促癌作用。
Suprabasin is a recently identified oncoprotein that is upregulated in multiple cancers. However, the clinical significance and biological role of suprabasin in human esophageal squamous cell carcinoma (ESCC) remains unclear. In the current study, we reported that suprabasin was markedly overexpressed in ESCC cell lines and tissues at both mRNA and protein levels and this was associated with advanced clinical stage, tumor-nodes-metastasis (TNM) classification, histological differentiation, tumor size and poorer survival. Furthermore, we found that both proliferation and tumorigenicity of ESCC cells were significantly induced by suprabasin overexpression, but inhibited by suprabasin knock-down. Moreover, we demonstrated that upregulation of suprabasin activated the Wnt/β-catenin signaling pathway and led to nuclear localization of β-catenin and upregulation of Cyclin D1 and c-Myc. Together, our results suggest that suprabasin plays an important oncogenic role in promoting proliferation and tumorigenesis of ESCC.