Combining Various Methods to Assess Insulin Sensitivity in Nonobese Rat after Sleeve Gastrectomy
Combining Various Methods to Assess Insulin Sensitivity in Nonobese Rat after Sleeve Gastrectomy
复制标题
结合多种方法评估非肥胖大鼠袖状胃切除术后的胰岛素敏感性
DOI：
10.1055/s-0043-125315
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发表时间：
2018
影响因子：
1.8
通讯作者：
Sanyuan Hu
中科院分区：
文献类型：
--
作者：
Dong Sun;Yanmin Wang;Meng Wei;Zongli Zhang;Sanyuan Hu
背景袖状胃切除术（SG）手术已被证明可以提高胰岛素敏感性并维持抗糖尿病作用。我们的目的是联合使用多种方法测量胰岛素敏感性，并探讨SG对术后早期和远期肝脏和外周胰岛素敏感性的影响。方法将30只11周龄雄性Goto-Kakizaki大鼠分为SG组、假手术SG（SOSG）和对照组。术前和术后观察36周。术前、术后2周、36周采用胰岛素耐量试验（ITT）和胰岛素抵抗稳态评估模型指数（HOMA-IR）测定胰岛素抵抗；术前及术后2周、36周进行丙酮酸激发试验（PCT）评估糖异生能力，反映肝脏胰岛素敏感性；术前及术后2、36周进行高胰岛素正常血糖钳夹（HIEC），计算基础和稳态时的内源性肝葡萄糖产生量（HGP），用于评价肝脏胰岛素敏感性；计算稳态时的外源性葡萄糖输注率（GIR），用于评价外周胰岛素敏感性。 与假手术组和对照组大鼠相比，SG组大鼠1）术后2周和36周的AUCITT、HOMA-IR和AUCPCT值显着降低，2）术后2周基础态HGP较低，但稳态GIR没有降低，3）术后36周基础和稳态HGP以及稳态GIR显着不同。此外，SG组大鼠术后2周和36周的基础和稳态HGP以及稳态GIR均存在显着差异。结论本研究联合采用多种技术探讨SG后大鼠的胰岛素敏感性。结果表明，SG 时间依赖性地改善肝脏和外周胰岛素敏感性。
BackgroundSleeve gastrectomy (SG) procedure has been proved to improve insulin sensitivity and sustain anti-diabetic effects. Our aim is to co-use several methods to measure insulin sensitivity and investigate the effect of SG on hepatic and peripheral insulin sensitivity at early and long-term stages of postoperation.MethodsThirty 11-week-old male Goto-Kakizaki rats were divided into SG, sham-operated SG (SOSG), and control groups. They were observed before operation and for 36 weeks of postoperation. Insulin tolerance test (ITT) and homeostasis model of assessment for insulin resistance index(HOMA-IR)were used to measure insulin resistance before operations and at 2 and 36 weeks of postoperation; Pyruvate challenge test (PCT) was administrated to assess the gluconeogenesis capability in order to reflect hepatic insulin sensitivity before operation and at 2 and 36 weeks of postoperation; Hyperinsulinemic euglycemic clamps (HIEC) was conducted before operation and at 2 and 36 weeks of postoperation to calculate the endogenous hepatic glucose production (HGP) at the basal and steady-state for evaluation of hepatic insulin sensitivity, and calculate the exogenous glucose infusion rate (GIR) at the steady-state for evaluation of peripheral insulin sensitivity.ResultsThe data showed that compared with rats in the sham and control groups, rats in SG group had 1) significantly lower AUCITT, HOMA-IR and AUCPCTvalues at 2 and 36 weeks of postoperation, 2) lower basal state HGP, but not steady-state GIR at 2 weeks of postoperation, and 3) significantly different basal and steady-state HGP and steady-state GIR at 36 weeks of postoperation. In addition, the basal and steady-state HGP and the steady-state GIR were significantly different between rats in SG group at 2 and 36 weeks of postoperation.ConclusionsThis study explored insulin sensitivity of rats after SG by jointly using a variety of techniques. The results showed that SG time-dependently improved the hepatic and peripheral insulin sensitivity.