First Cultivation and Ecological Investigation of a Bacterium Affiliated with the Candidate Phylum OP5 from Hot Springs
First Cultivation and Ecological Investigation of a Bacterium Affiliated with the Candidate Phylum OP5 from Hot Springs
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DOI：
10.1128/aem.01351-08
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发表时间：
2008-09
影响因子：
4.4
通讯作者：
K. Mori;M. Sunamura;K. Yanagawa;J. Ishibashi;Youko Miyoshi;Takao Iino;Ken-ichiro Suzuki;T. Urabe
中科院分区：
文献类型：
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作者：
K. Mori;M. Sunamura;K. Yanagawa;J. Ishibashi;Youko Miyoshi;Takao Iino;Ken-ichiro Suzuki;T. Urabe
系统发育类群OP5最初是在黄石国家公园的温泉中发现的，并被认为是一个未培养的门；随后采用文化独立方法对该组进行分析。最近，从日本的温泉中获得了一种新的嗜热化学异养丝状细菌，通过各种分离程序进行了富集。系统发育分析表明，该分离物与主要由嗜热和中温厌氧环境克隆构建的OP5门密切相关。在细菌领域，谱系似乎在门水平上是独立的。因此，我们设计了针对OP5门的16S rRNA基因引物，并采用real-time PCR方法进行了定量场分析。因此，在部分温泉样品中检测到OP5门16S rRNA基因，其相对丰度在检测到的原核生物中占0.2% ~ 1.4%。上述分离物及其环境无性系的生理特征表明，它们是自然界中参与硫循环的清道夫。
ABSTRACT The phylogenetic group termed OP5 was originally discovered in the Yellowstone National Park hot spring and proposed as an uncultured phylum; the group was afterwards analyzed by applying culture-independent approaches. Recently, a novel thermophilic chemoheterotrophic filamentous bacterium was obtained from a hot spring in Japan that was enriched through various isolation procedures. Phylogenetic analyses of the isolate have revealed that it is closely related to the OP5 phylum that has mainly been constructed with the environmental clones retrieved from thermophilic and mesophilic anaerobic environments. It appears that the lineage is independent at the phylum level in the domain Bacteria. Therefore, we designed a primer set for the 16S rRNA gene to specifically target the OP5 phylum and performed quantitative field analysis by using the real-time PCR method. Thus, the 16S rRNA gene of the OP5 phylum was detected in some hot-spring samples with the relative abundance ranging from 0.2% to 1.4% of the prokaryotic organisms detected. The physiology of the above-mentioned isolate and the related environmental clones indicated that they are scavengers contributing to the sulfur cycle in nature.