Adaptive optics for enhanced signal in CARS microscopy
Adaptive optics for enhanced signal in CARS microscopy
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DOI：
10.1364/oe.15.018209
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发表时间：
2007-12-24
期刊：
影响因子：
3.8
通讯作者：
Xie, X. S.
中科院分区：
文献类型：
--
作者：
Wright, A. J.;Poland, S. P.;Xie, X. S.
我们报告了使用自适应光学与相干反斯托克斯拉曼散射（CARS）显微镜进行基于分子振动光谱的无标记深层组织成像。该装置采用可变形膜镜和随机搜索优化算法来提高大样本深度下的信号强度和图像质量。我们展示了校正测试样本以及肌肉组织中系统和样本引起的畸变的能力，以增强 CARS 信号。组合系统和样品引起的像差校正使700μm深度的测试样品的信号平均增加了约3倍，而对于260μm深度的肌肉组织，信号平均增加了约6倍。自适应光学器件提供的增强信号和更高穿透深度将增强 CARS 显微镜作为体内和原位生物医学成像方式的能力。 (c) 2007 年美国光学学会。
We report the use of adaptive optics with coherent anti-Stokes Raman scattering (CARS) microscopy for label-free deep tissue imaging based on molecular vibrational spectroscopy. The setup employs a deformable membrane mirror and a random search optimization algorithm to improve signal intensity and image quality at large sample depths. We demonstrate the ability to correct for both system and sample-induced aberrations in test samples as well as in muscle tissue in order to enhance the CARS signal. The combined system and sample- induced aberration correction increased the signal by an average factor of similar to 3x for the test samples at a depth of 700 mu m and similar to 6x for muscle tissue at a depth of 260 mu m. The enhanced signal and higher penetration depth offered by adaptive optics will augment CARS microscopy as an in vivo and in situ biomedical imaging modality. (c) 2007 Optical Society of America.