QUANTIFICATION OF INDIVIDUAL SUBGENOMIC MESSENGER-RNA SPECIES DURING REPLICATION OF THE CORONAVIRUS TRANSMISSIBLE GASTROENTERITIS VIRUS
QUANTIFICATION OF INDIVIDUAL SUBGENOMIC MESSENGER-RNA SPECIES DURING REPLICATION OF THE CORONAVIRUS TRANSMISSIBLE GASTROENTERITIS VIRUS
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DOI：
10.1016/0168-1702(94)00108-o
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发表时间：
1995-05-01
期刊：
影响因子：
5
通讯作者：
BRITTON, P
中科院分区：
文献类型：
--
作者：
HISCOX, JA;CAVANAGH, D;BRITTON, P
采用生物素标记寡核苷酸的方法分离冠状病毒传染性胃肠炎病毒（TGEV）亚基因组mRNA，研究其在复制周期的早、中、晚3个阶段的mRNA表达量。TGEV mRNA 6编码N蛋白，被观察到是整个复制周期中最丰富的种类。在感染后12 h，mRNA 6与其他mRNA的比例分别为1：0.11（mRNA 2）、1：0.16（mRNA 3和4）和1：0.37（mRNA 5）。所有的mRNA种类的差异调节整个复制周期，虽然积累的mRNA 4，5和6，但不是mRNA 3的速率，增加显着接近复制周期结束。在所用的系统中未检测到mRNA 7。在复制周期中的任何时间，合成的每种mRNA的量与前导序列3'末端和基因组RNA上的转录相关序列之间的潜在碱基配对程度之间没有可观察到的相关性。这表明碱基配对的程度不是参与控制亚基因组mRNA合成的唯一因素。
A biotinylated-oligonucleotide-based method was used to isolate the subgenomic mRNAs of the coronavirus transmissible gastroenteritis virus (TGEV) to investigate the amounts of the mRNAs produced at early, middle and late times in the replication cycle. TGEV mRNA 6, which encodes the N protein, was observed to be the most abundant species throughout the replication cycle. The ratios of mRNA 6 to the other mRNAs were 1:0.11 (mRNA 2), 1:0.16 (mRNAs 3 and 4) and 1:0.37 (mRNA 5) at 12 h post-infection. All the mRNA species were differentially regulated throughout the replication cycle, although the rate of accumulation of mRNAs 4, 5 and 6, but not mRNA 3, increased markedly towards the end of the replication cycle. mRNA 7 was not detected in the system used. There was no observable correlation between the amounts of each mRNA synthesised and the potential degree of base pairing between the 3' end of the leader sequence and the transcription associated sequences on the genomic RNA at any time during the replication cycle. This indicates that the extent of base pairing was not the only factor involved in the control of subgenomic mRNA synthesis.