Optimal parameters of transcorneal electrical stimulation (TES) to be neuroprotective of axotomized RGCs in adult rats
Optimal parameters of transcorneal electrical stimulation (TES) to be neuroprotective of axotomized RGCs in adult rats
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DOI：
10.1016/j.exer.2009.11.002
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发表时间：
2010-02-01
影响因子：
3.4
通讯作者：
Fujikado, Takashi
中科院分区：
文献类型：
--
作者：
Morimoto, Takeshi;Miyoshi, Tomomitsu;Fujikado, Takashi
我们以前的研究表明，经角膜电刺激（TES）促进大鼠轴突切断的视网膜神经节细胞（RGCs）的存活。然而，TES的参数之间的关系和TES对轴突切断的RGCs的神经保护作用尚不清楚。在本研究中，我们确定TES的神经保护作用是否受到TES参数的影响。成年雄性Wistar大鼠在切断左侧视神经（ON）后立即接受TES。系统地改变TES的脉冲持续时间、电流强度、频率、波形和会话次数。在ON切断后7天或14天，对视网膜的改变进行组织学检查。最佳的神经保护参数是脉冲持续时间为1和2 ms/相（P < 0.001，每个），电流强度为100和200 μ A（P < 0.05，每个），刺激频率为1，5和20 Hz（P < 0.001，分别）。TES作用30 min以上才能产生神经保护作用（P < 0.001）。无脉冲间隔的对称脉冲最有效（P < 0.001）。在ON切断后14天，重复TES比单次TES具有更好的神经保护作用（P < 0.001）。本研究结果提示TES对切断轴突的大鼠RGCs的神经保护作用存在一定的最佳参数范围。这些值将为TES在不同视网膜和视神经疾病患者中的使用提供指导。(C)2009爱思唯尔有限公司保留所有权利。
We previously showed that transcorneal electrical stimulation (TES) promoted the survival of axotomized retinal ganglion cells (RGCs) of rats. However the relationship between the parameters of TES and the neuroprotective effect of TES on axotomized RGCs was unclear. In the present study, we determined whether the neuroprotective effect of TES is affected by the parameters of TES. Adult male Wistar rats received TES just after transection of the left optic nerve (ON). The pulse duration, current intensity, frequency, waveform, and numbers of sessions of the TES were changed systematically. The alterations of the retina were examined histologically seven days or fourteen days after the ON transection. The optimal neuroprotective parameters were pulse duration of I and 2 ms/phase (P < 0.001, each), current intensity of 100 and 200 mu A (P < 0.05, each), and stimulation frequency of 1, 5, and 20 Hz (P < 0.001, respectively). More than 30 min of TES was necessary to have a neuroprotective effect (P < 0.001). Symmetric pulses without an inter-pulse interval were most effective (P < 0.001). Repeated TES was more neuroprotective than a single TES at 14 days after ON transection (P < 0.001). Our results indicate that there is a range of optimal neuroprotective parameters of TES for axotomized RGCs of rats. These values will provide a guideline for the use of TES in patients with different retinal and optic nerve diseases. (C) 2009 Elsevier Ltd. All rights reserved.