Study on the PEG-based microwave-assisted extraction of flavonoid compounds from persimmon leaves
Study on the PEG-based microwave-assisted extraction of flavonoid compounds from persimmon leaves
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基于PEG的微波辅助提取柿叶黄酮类化合物的研究
DOI：
10.1002/jssc.201200495
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发表时间：
2012-12-01
影响因子：
3.1
通讯作者：
Zhang, Zhi-Qi
中科院分区：
文献类型：
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作者：
Liu, Lei;Liu, Rui-Lin;Zhang, Zhi-Qi
以柿叶为原料，研究了微波辅助提取柿叶中黄酮类化合物的方法。以柿叶中主要活性成分槲皮素和山奈酚的色谱峰面积为指标，评价了柿叶总黄酮的提取效率。采用响应面分析法确定最佳提取工艺参数组合。结果表明，微波功率为525 W，液固比为17：1 mL/g，PEG水溶液浓度为60%w/w为最佳工艺参数。提取动力学分析表明，槲皮素、山奈酚和总黄酮的含量随着提取时间的增加而增加，提取时间为2025 min时，提取动力学分析表明，提取时间为2025 min时，槲皮素、山奈酚和总黄酮的含量随着提取时间的增加而增加，提取时间为2025 min时，槲皮素、山奈酚和总黄酮的含量均下降。在最佳条件下，槲皮素，山奈酚，总黄酮含量分别为1.20 +/- 0.05，0.64 +/- 0.11，和16.90 +/- 0.06 mg/g。结果表明，与乙醇微波辅助提取法、乙醇微波辅助提取法和PEG微波辅助提取法相比，PEG微波辅助提取法对柿叶黄酮类化合物的提取效率更高。总体而言，基于PEG的MAE代表了从传统中药中提取生物活性物质的有效选择。
A method for PEG-based microwave-assisted extraction (MAE) of flavonoid compounds from persimmon leaves has been successfully developed. The extraction efficiency of total flavonoid content was evaluated by the chromatographic peak areas of quercetin and kaempferol, which are two bioactive components typically found in persimmon leaves. The best combination of extraction parameters was obtained with response surface methodology. A microwave power of 525 W, liquid to solid ratio of 17:1 mL/g, and PEG aqueous solution concentration of 60% w/w were identified as the optimum parameters. Extraction dynamics analysis indicated that the quercetin, kaempferol, and total flavonoid contents were rising with increasing extraction time up to 2025 min, from which point onwards they all decreased. Under the optimum conditions, quercetin, kaempferol, and total flavonoid contents obtained from the sample were 1.20 +/- 0.05, 0.64 +/- 0.11, and 16.90 +/- 0.06 mg/g, respectively. Compared with ethanol-based MAE, and ethanol-based and PEG-based ultrasonic-assisted extractions, PEG-based MAE had higher efficiency for the extraction of flavonoid compounds from persimmon leaves. Overall, PEG-based MAE represents an efficient choice for the extraction of bioactive substances from traditional Chinese medicines.