Prognostic significance of VEGF expression in correlation with COX-2, microvessel density, and clinicopathological characteristics in human gastric carcinoma
Prognostic significance of VEGF expression in correlation with COX-2, microvessel density, and clinicopathological characteristics in human gastric carcinoma
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DOI：
10.1245/s10434-007-9484-7
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发表时间：
2007-10-01
影响因子：
3.7
通讯作者：
Sugihara, Kenichi
中科院分区：
文献类型：
--
作者：
Kolev, Yanislav;Uetake, Hiroyuki;Sugihara, Kenichi
背景：许多研究表明，血管生成在肿瘤的发生、发展过程中起着重要作用。血管内皮生长因子（VEGF）具有强的血管生成活性，而环氧合酶-2（考克斯-2）通过调节包括VEGF在内的血管生成因子的产生来支持血管生成。本研究旨在探讨VEGF、考克斯-2和CD 34在胃癌组织中的表达及其与临床病理因素的关系，并探讨其与胃癌预后的关系。用CD 34免疫染色评估肿瘤微血管密度。研究VEGF、考克斯-2、CD 34的表达与各种临床病理因素的关系。结果：考克斯-2和VEGF的阳性表达率分别为36.7%和50.3%。VEGF与考克斯-2、CD 34呈正相关。VEGF表达与浸润深度、淋巴结转移、淋巴结和静脉浸润以及肿瘤、淋巴结和转移系统分期有关。VEGF阳性患者的无病生存率（64.9%对81.3%）和总生存率（58.3%对76.9%）远低于VEGF阴性患者。在多因素分析中，只有肿瘤的位置，浸润深度和淋巴结转移被证明是独立的预后因素。结论：VEGF表达与血管生成和肿瘤进展，是一个有价值的预后因素在胃癌患者。
Background: Many studies have shown that angiogenesis plays an important role in the process of cancer development and progression. Vascular endothelial growth factor (VEGF) has a potent angiogenic activity, and cyclooxygenase-2 (COX-2) supports angiogenesis by regulated production of angiogenic factors, including VEGF. The purpose of this study was to examine the expression of VEGF in combination with COX-2 and CD34, their correlation with various clinicopathological factors, and their prognostic significance in human gastric carcinoma.Methods: Specimens from 169 patients with different grade and stage gastric carcinoma were investigated by immunohistochemistry for COX-2 and VEGF expression. Tumor microvessel density was assessed with CD34 immunostaining. Correlations between the expression of VEGF, COX-2, CD34, and various clinicopathological factors were studied. The effect of these proteins on patient survival was determined.Results: COX-2 and VEGF were positively expressed in 36.7% and 50.3% of the patients, respectively. Positive correlation was found between VEGF and COX-2 and between VEGF and CD34. VEGF expression was correlated with depth of invasion; metastatic lymph nodes; lymphatic and venous invasion; and tumor, node, metastasis system stage. Patients with positive staining for VEGF showed far lower disease-free (64.9% vs. 81.3%) and overall (58.3% vs. 76.9%) survival rates than VEGF-negative patients. In multivariate analysis, only tumor location, depth of invasion, and lymph node metastasis were shown to be independent prognostic factors.Conclusions: VEGF expression correlates with angiogenesis and tumor progression and is a valuable prognostic factor in patients with gastric carcinoma.