Paeoniflorin improves menopause depression in ovariectomized rats under chronic unpredictable mild stress.
Paeoniflorin improves menopause depression in ovariectomized rats under chronic unpredictable mild stress.
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发表时间：
2015-04
影响因子：
0.1
通讯作者：
Hongli Huang;Juan Zhao;Lin Jiang;Yuan Xie;Yanqiu Xia;Rong Lv;Li-jun Dong
中科院分区：
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作者：
Hongli Huang;Juan Zhao;Lin Jiang;Yuan Xie;Yanqiu Xia;Rong Lv;Li-jun Dong
牡丹苷已被证明能有效地缓解神经损伤和减轻抑郁。目前尚不清楚其是否可用于治疗更年期抑郁症，因此，本研究通过切除卵巢联合长期慢性不可预知的应激建立更年期抑郁症动物模型。采用卵巢摘除的方法建立更年期抑郁动物模型。给SD大鼠灌胃10 mg/kg的芍药苷，连续2周。氟西汀(2.5 mg/kg)作为阳性对照。蔗糖溶液消耗实验、开场实验、实时荧光定量聚合酶链式反应和免疫印迹实验结果表明，芍药苷增加了小鼠对蔗糖溶液的消耗、自主行为和5-HT1AR基因和蛋白的表达。芍药苷可降低更年期抑郁症大鼠促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)、皮质醇(CORT)及5-HT2AR的mRNA和蛋白表达。这些结果表明，芍药苷不调节更年期抑郁症大鼠脑内5-HT1AR的表达，但下调5-HT2AR的表达，从而发挥抗抑郁作用。
Paeoniflorin has been shown to effectively relieve neurologic impairments and lessen depression. It remains poorly understood whether it can be used to treat menopause depression; therefore, in the present study, animal model of menopause depression were established by resecting the ovaries in combined with long-term chronic unpredictable stress. Animal model of menopause depression was established by ovariectomy. Sprague-Dawley rats were given 10 mg/kg of paeoniflorin by gastrogavage for 2 weeks. Fluoxetine (2.5 mg/kg) served as a positive control. Sucrose solution consumption test, open-field test, real-time PCR and western blot results demonstrated that paeoniflorin increased sucrose solution consumption, voluntary behaviors and 5-HT1AR mRNA and protein expression. Paeoniflorin decreased the levels of corticotropin releasing hormone (CRH), adrenocorticotropic hormone (ACTH), cortisol (CORT) and 5-HT2AR mRNA and protein expression in rats with menopause depression. These results indicate that paeoniflorin unregulated 5-HT1AR expression, but downregulated 5-HT2AR expression in brains of rats with menopause depression, and thus exert antidepressant effects.