Phenolic acid-induced phase separation and translation inhibition mediate plant interspecific competition
Phenolic acid-induced phase separation and translation inhibition mediate plant interspecific competition
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DOI：
10.1038/s41477-023-01499-6
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发表时间：
2023-08
期刊：
影响因子：
18
通讯作者：
Zhouli Xie;Shuai Zhao;Y. Li;Yuhua Deng;Yabo Shi;Xiaoyuan Chen;Yue Li;Haiwei Li;Changtian Chen;Xingwei Wang;Enhui Liu;Yuchen Tu;Peng Shi;Jinjin Tong;Emilio Gutiérrez-Beltrán;Jiayu Li;P. Bozhkov;Weiqiang Qian;Mian Zhou;Wen Wang
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文献类型：
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作者：
Zhouli Xie;Shuai Zhao;Y. Li;Yuhua Deng;Yabo Shi;Xiaoyuan Chen;Yue Li;Haiwei Li;Changtian Chen;Xingwei Wang;Enhui Liu;Yuchen Tu;Peng Shi;Jinjin Tong;Emilio Gutiérrez-Beltrán;Jiayu Li;P. Bozhkov;Weiqiang Qian;Mian Zhou;Wen Wang
供体植物分泌的酚酸（PA）抑制其易感植物邻居的生长。然而，如何结构多样的合奏的PA被认为是由植物介导种间竞争仍然是一个谜。在这里，我们表明，植物胁迫颗粒（SG）标记，RNA结合蛋白47 B（RBP 47 B），是一个传感器的多胺在拟南芥。水杨酸、4-羟基苯甲酸、原儿茶酸等多聚体直接与RBP 47 B结合，促进其相分离，引发SG形成，并伴有全面的翻译抑制。水杨酸诱导的全局翻译抑制依赖于RBP 47家族成员。RBP 47调节蛋白质组而不是SG的绝对量。Therbp 47四倍体突变体在共培养生态系统中对PA混合物的抑制作用以及对富含PA的水稻的抑制作用的敏感性降低。在这篇文章中，我们确定了长期追求的PA传感器RBP 47 B，并说明PA诱导的SG介导的翻译抑制是PA感知机制之一。
Phenolic acids (PAs) secreted by donor plants suppress the growth of their susceptible plant neighbours. However, how structurally diverse ensembles of PAs are perceived by plants to mediate interspecific competition remains a mystery. Here we show that a plant stress granule (SG) marker, RNA-BINDING PROTEIN 47B (RBP47B), is a sensor of PAs inArabidopsis. PAs, including salicylic acid, 4-hydroxybenzoic acid, protocatechuic acid and so on, directly bind RBP47B, promote its phase separation and trigger SG formation accompanied by global translation inhibition. Salicylic acid-induced global translation inhibition depends on RBP47 family members. RBP47s regulate the proteome rather than the absolute quantity of SG. Therbp47quadruple mutant shows a reduced sensitivity to the inhibitory effect of the PA mixture as well as to that of PA-rich rice when tested in a co-culturing ecosystem. In this Article, we identified the long sought-after PA sensor as RBP47B and illustrated that PA-induced SG-mediated translational inhibition was one of the PA perception mechanisms.