Short repeats cause heterogeneity at genomic terminus of bovine herpesvirus 1
Short repeats cause heterogeneity at genomic terminus of bovine herpesvirus 1
复制标题
短重复导致牛疱疹病毒 1 型基因组末端的异质性
DOI：
10.1128/jvi.58.1.43-49.1986
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发表时间：
1986
影响因子：
5.4
通讯作者：
H. Buhk
中科院分区：
文献类型：
--
作者：
W. Hammerschmidt;H. Ludwig;H. Buhk
不同的牛疱疹病毒1型株的基因组分析显示UL末端HindIII片段大小不同（从2.4到2.8个内切酶）。该片段多态性发生在野生型分离株的DNA中，在高度传代的，无致病性的组织培养衍生物中，以及在空斑纯化的亚株中。这种异质性是由于包含碱基序列5 '-GCTCCTCCTCCCTC-3'的14碱基对串联重复的拷贝数的变化，该碱基序列也存在于单纯疱疹病毒1型、爱泼斯坦-巴尔病毒和相关人类细胞DNA的其他短重复序列中，但存在一些差异。此外，串联重复序列阵列位于病毒基因组左端附近，可能在功能上参与病毒复制。
Analysis of the genomes of different bovine herpesvirus 1 strains revealed a UL terminal HindIII fragment differing in size (from 2.4 to 2.8 kilobases). This fragment polymorphism occurred in the DNA of a wild-type isolate, in highly passaged, apathogenic tissue culture derivatives, and in plaque-purified substrains. This heterogeneity was due to variations in the copy number of a 14-base-pair tandem repeat comprising the base sequence 5'-GCTCCTCCTCCCTC-3', which also exists, with some differences, in other short reiteration sequences of herpes simplex virus type 1, Epstein-Barr virus, and related human cellular DNA. Furthermore, the tandem repeat array was located in close proximity to the left end of the viral genome and may functionally be involved in viral replication.