TYPE BETA-TRANSFORMING GROWTH-FACTOR IS AN INHIBITOR OF MYOGENIC DIFFERENTIATION
TYPE BETA-TRANSFORMING GROWTH-FACTOR IS AN INHIBITOR OF MYOGENIC DIFFERENTIATION
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DOI：
10.1073/pnas.83.21.8206
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发表时间：
1986-11-01
影响因子：
11.1
通讯作者：
NADALGINARD, B
中科院分区：
文献类型：
--
作者：
MASSAGUE, J;CHEIFETZ, S;NADALGINARD, B
我们研究了β型的效果。TGF- β对骨骼肌成肌细胞分化的影响。TGF度量。有力地；有力地；（10 pM）阻止已建立的细胞系和原代培养的大鼠和鸡胚成肌细胞融合成多核肌管。TGF-. β对形态分化的抑制作用。与抑制肌肉特异性mrna和蛋白质的表达，强烈诱导细胞外基质I型胶原和纤维连接蛋白，以及处理后的成肌细胞聚集成密集的多层阵列或簇的明显趋势相关。TGF- β去除后可恢复肌源性分化。从媒介。TGF- β的作用时间检测。表明成肌细胞随机到达一个点，超过这个点，它们对TGF-. β的抑制作用变得不敏感。这种肌母细胞对TGF-抑制作用的抵抗。与TGF- β的数量或亲和力的任何可测量的变化无关。这些细胞中的受体。结果表明TGF-. β。是一种有效的肌肉发生抑制剂，可以调节体内肌肉发育。
We have investigated the effect of type .beta. transforming growth factor (TGF-.beta.) on the differentiation of skeletal muscle myoblasts. TGF-.beta. potently (ID50 .apprxeq. 10 pM) prevents established cell lines and primary cultures of rat and chicken embryo myoblasts from fusing into multinucleated myotubes. Inhibition of morphological differentiation by TGF-.beta. correlates with inhibition of the expression of muscle-specific mRNAs and proteins, strong induction of extracellular matrix type I collagen and fibronectin, and a marked tendency of the treated myoblasts to aggregate into densely multilayered arrays or clusters. Myogenic differentiation can resume after removal of TGF-.beta. from the medium. Examination of the time of action of TGF-.beta. shows that myoblasts stochastically reach a point beyond which they become insensitive to the inhibitory action of TGF-.beta.. This resistance of committed myoblasts to the inhibitory action of TGF-.beta. is not associated with any measurable change in the number or affinity of TGF-.beta. receptors in those cells. The results indicate that TGF-.beta. is a potent inhibitor of myogenesis and may regulate muscle development in vivo.