An EPFL peptide signaling pathway promotes stamen elongation via enhancing filament cell proliferation to ensure successful self-pollination in Arabidopsis thaliana
An EPFL peptide signaling pathway promotes stamen elongation via enhancing filament cell proliferation to ensure successful self-pollination in Arabidopsis thaliana
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DOI：
10.1111/nph.18806
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发表时间：
2023-02-28
期刊：
影响因子：
9.4
通讯作者：
Meng, Xiangzong
中科院分区：
文献类型：
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作者：
He, Yunxia;He, Xiaomeng;Meng, Xiangzong
雄蕊花丝的适当伸长是植物自花授粉和繁殖的必要条件。几种植物激素如茉莉酸和赤霉素在控制花丝伸长中起重要作用，但参与这一发育过程的其他内源信号尚不清楚。我们在这里报告的三个表皮图案形成因子样（EPFL）家族肽，EPFL 4，EPFL 5和EPFL 6，多余的行为，促进雄蕊丝伸长通过增强丝细胞增殖拟南芥。EPFL 4 -6基因的敲除导致花丝细胞增殖缺陷导致花丝缩短，导致授粉失败和雄性不育。进一步的遗传和生化分析表明，ERECTA家族和体细胞胚胎发生受体激酶（SERK）家族RLK形成受体复合物，以感知EPFL 4 -6肽并促进丝细胞增殖。此外，基于功能丧失和获得的遗传分析，丝裂原活化蛋白激酶级联MKK 4/MKK 5-MPK 6被证明在EPFL 4 -6的下游起作用，以正向调节雄蕊丝中的细胞增殖。总之，本研究揭示了由EPFL 4 -6肽配体、ERECTA-SERK受体复合物和下游MKK 4/MKK 5-MPK 6级联组成的EPFL肽信号通路通过增强花丝细胞增殖来促进雄蕊花丝伸长，以确保拟南芥中成功的自花授粉和正常的育性。
Proper stamen filament elongation is essential for plant self-pollination and reproduction. Several phytohormones such as jasmonate and gibberellin play important roles in controlling filament elongation, but other endogenous signals involved in this developmental process remain unknown. We report here that three EPIDERMAL PATTERNING FACTOR-LIKE (EPFL) family peptides, EPFL4, EPFL5 and EPFL6, act redundantly to promote stamen filament elongation via enhancing filament cell proliferation in Arabidopsis thaliana. Knockout of EPFL4-6 genes led to shortened filaments due to defective filament cell proliferation, resulting in pollination failure and male sterility. Further genetic and biochemical analyses indicated that the ERECTA family and the SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) family RLKs form receptor complexes to perceive EPFL4-6 peptides and promote filament cell proliferation. Moreover, based on both loss- and gain-of-function genetic analyses, the mitogen-activated protein kinase cascade MKK4/MKK5-MPK6 was shown to function downstream of EPFL4-6 to positively regulate cell proliferation in stamen filaments. Together, this study reveals that an EPFL peptide signaling pathway composed of the EPFL4-6 peptide ligands, the ERECTA-SERK receptor complexes and the downstream MKK4/MKK5-MPK6 cascade promotes stamen filament elongation via enhancing filament cell proliferation to ensure successful self-pollination and normal fertility in Arabidopsis.