Changes in Allele-Specific Association of Histone Modifications at the Imprinting Control Regions During Mouse Preimplantation Development
Changes in Allele-Specific Association of Histone Modifications at the Imprinting Control Regions During Mouse Preimplantation Development
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DOI：
10.1002/dvg.20541
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发表时间：
2009-09-01
期刊：
影响因子：
1.5
通讯作者：
Ogura, Atsuo
中科院分区：
文献类型：
--
作者：
Kim, Jin-Moon;Ogura, Atsuo
组蛋白修饰的等位基因特异性关联在印迹基因的调控区被观察到，并且已经被建议作为单等位基因基因表达的表观遗传标记，与DNA的等位基因CpG甲基化一起沿着。尽管印迹基因中的父母来源特异性表观遗传状态被认为是在植入前发育期间建立的，但由于可用于分析的材料有限，因此对这一时期组蛋白修饰的等位基因特异性知之甚少。在这项研究中，我们首次揭示了等位基因富集组蛋白修饰和变体的历史在印记控制区（ICR）的四细胞囊胚期植入前胚胎通过使用载体染色质免疫沉淀和序列多态性分析的免疫沉淀DNA。我们发现组蛋白H3赖氨酸9二甲基化在ICR的印迹等位基因的相对富集，并获得的结果表明，在ICR的组蛋白修饰是在后期植入前阶段建立的。创世纪47：611-616，2009。(C)2009 Wiley-Liss，Inc.
Allele-specific association of histone modification is observed at the regulatory region of imprinted genes and has been suggested to work as an epigenetic marker for monoallelic gene expression, along with the allelic CpG methylation of DNA. Although the parent-origin-specific epigenetic status in imprinted genes is thought to be established during preimplantation development, little is known about the allelic specificity of histone modifications during this period because of the limited volume of material available for analysis. In this study, we first revealed the allelic enrichment of histone modifications and variant histories at the imprinting control regions (ICRs) of four-cell to blastocyst stage preimplantation embryos by using carrier chromatin immunoprecipitation and sequence polymorphism analysis of immunoprecipitated DNA. We found relative enrichment of histone H3 lysine 9 dimethylation at the imprinted alleles of ICRs and obtained the results suggesting that histone modifications at ICRs are established during a late preimplantation stage. genesis 47:611-616, 2009. (C) 2009 Wiley-Liss, Inc.