The ADAXIALIZED LEAF1 gene functions in leaf and embryonic pattern formation in rice
The ADAXIALIZED LEAF1 gene functions in leaf and embryonic pattern formation in rice
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DOI：
10.1016/j.ydbio.2009.07.042
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发表时间：
2009-10-15
影响因子：
2.7
通讯作者：
Nagato, Yasuo
中科院分区：
文献类型：
--
作者：
Hibara, Ken-ichiro;Obara, Mari;Nagato, Yasuo
叶原基中的近轴-远轴轴被认为是首先建立的，是叶片沿内侧轴扩展所必需的。为了了解叶片发育中的轴信息，我们分离了水稻中形成背面卷叶的adl1突变体。ADI1叶片上覆盖着泡状细胞，通常只分布在叶片的正面。与正面雪叶突变体的adl1双突变体相比，adl1突变体的白化细胞出现在叶肉组织的背面，这表明adl1叶片在表皮和叶肉组织中都表现出黄化。在成熟叶片中，HD-ZIPIII基因在adl1突变体中的表达增强。但不是在年轻的原始体或SAM中。这表明ADL1可能不直接参与决定初始叶的极性，而与轴信息的维持有关。ADO编码一种与玉米缺陷型KERNEL1同源的植物特异性钙蛋白酶样半胱氨酸蛋白酶。此外，我们鉴定了adl1突变体的中等等位基因和强等位基因，它们分别产生了无芽胚胎和球状滞留胚胎，糊粉层丢失。我们认为，ADL1通过促进表皮的正常发育，在叶片和胚胎的模式形成中发挥重要作用。(C)2009 Elsevier Inc.保留所有权利。
The adaxial-abaxial axis in leaf primordia is thought to be established first and is necessary for the expansion of the leaf lamina along the mediolateral axis. To understand axis information in leaf development, we isolated the adaxialized leaf1 (adl1) mutant in rice, which forms abaxially rolled leaves. adi1 leaves are covered with bulliform-like cells, which are normally distributed only on the adaxial surface. An adl1 double mutant with the adaxially snowy leaf mutant, which has albino cells that specifically appear in the abaxial mesophyll tissue, indicated that adl1 leaves show adaxialization in both epidermal and mesophyll tissues. The expression of HD-ZIPIII genes in adl1 mutant increased in mature leaves. but not in the young primordia or the SAM. This indicated that ADL1 may not be directly involved in determining initial leaf polarity, but rather is associated with the maintenance of axis information. ADO encodes a plant-specific calpain-like cysteine proteinase orthologous to maize DEFECTIVE KERNEL1. Furthermore, we identified intermediate and strong alleles of the adl1 mutant that generate shootless embryos and globular-arrested embryos with aleurone layer loss, respectively. We propose that ADL1 plays an important role in pattern formation of the leaf and embryo by promoting proper epidermal development. (C) 2009 Elsevier Inc. All rights reserved.