CAPILLARY ELECTROPHORESIS WITH ELECTROCHEMICAL DETECTION EMPLOYING AN ON-COLUMN NAFION JOINT
CAPILLARY ELECTROPHORESIS WITH ELECTROCHEMICAL DETECTION EMPLOYING AN ON-COLUMN NAFION JOINT
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DOI：
10.1016/0021-9673(92)80299-a
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发表时间：
1992-02-28
期刊：
影响因子：
--
通讯作者：
WATANABE, N
中科院分区：
文献类型：
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作者：
OSHEA, TJ;GREENHAGEN, RD;WATANABE, N
介绍了一种利用Nafion管将毛细管区带电泳系统的检测端与电路隔离的柱上接头的构造和评价。接头使得能够执行电化学检测，而不会受到所施加的高电压的不利影响。该接头结构简单且耐用。采用碳纤维工作电极的电化学检测器表现出高的库仑效率，对苯二酚的检测限为6.10（-9）M或34.8阿莫尔。对于该化合物实现了约185000理论塔板的高效率。该系统进行了评估，用于检测苹果汁中的酚酸和脑匀浆中的萘-2，3-二甲醛衍生的氨基酸的测定。还证明了使用伏安法作为化合物验证的方法。
The construction and evaluation of an on-column joint utilizing Nafion tubing for the isolation of the electrical circuit from the detection end of a capillary zone electrophoresis system is described. The joint enables electrochemical detection to be performed without adverse effects from the applied high voltage. The joint is both simple to construct and durable. The electrochemical detector employing a carbon fiber working electrode exhibited high coulometric efficiencies and a detection limit of 6.10(-9) M or 34.8 amol for hydroquinone. A high efficiency, of the order of 185 000 theoretical plates, was achieved for this compound. This system was evaluated for the detection of phenolic acids in apple juice and for the determination of naphthalene-2,3-dicarboxaldehyde derivatized amino acids in a brain homogenate. The use of voltammetry as a method of compound verification was also demonstrated.