Effects of denervation upon receptor cell survival and basal cell proliferation in tuberous electroreceptor organs of a weakly electric fish.
Effects of denervation upon receptor cell survival and basal cell proliferation in tuberous electroreceptor organs of a weakly electric fish.
复制标题
去神经对弱电鱼结节性电感受器器官中受体细胞存活和基底细胞增殖的影响。
DOI：
10.1002/cne.903470406
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Zakon,HH
中科院分区：
文献类型：
--
作者：
Weisleder,P;Lu,Y;Zakon,HH
弱电鱼类产生电场，用于电子定位和通信。这些区域是由专门的感受器：结节器官探测到的。在本研究中，我们研究了去神经对受体细胞存活和祖细胞(基础)细胞增殖率的影响。切开棕色幽灵左侧眶下前外侧线神经，近端用蓖麻毒素浸泡以防止再生。在四组动物中，在失神经后1、2、3或4周每天注射两次细胞增殖标记物溴脱氧尿苷(BrdU)，持续2天。在完成BrdU注射程序后，从左侧(去神经)和右侧(完整)取出一块位于眼睛旋转腹侧的面颊皮肤，并进行光学显微镜或免疫细胞化学处理。我们的结果表明：(1)失神经后受体细胞进行性死亡和结节器官变性；(2)失神经和结节器官变性后，基底细胞的增殖随着时间的推移而稳步增加；(3)尽管去神经，一些增殖的基底细胞分化为受体细胞，但这些新的受体细胞最终死亡。这些结果表明，神经支配对于结节状电感受器细胞的存活是必不可少的，基底细胞的增殖速度受受体细胞健康、局部释放的因子或两者共同调节。©1994 Wiley-Liss，Inc.
Weakly electric fish generate electric fields for the purposes of electrolocation and communication. These fields are detected by specialized receptor organs: the tuberous organs. In the present study we investigated the effects of denervation upon receptor cell survival and progenitor (basal) cell proliferation rate. The left, infraorbital, anterior lateral line nerve of brown ghosts (Apteronotus leptorhynchus) was sectioned, and the proximal stump was dipped in ricin to prevent regrowth. In groups of four, the animals were given two daily injections of the cell proliferation marker bromodeoxyuridine (BrdU) for 2 days at 1, 2, 3, or 4 weeks following denervation. At the completion of the BrdU injection schedule, a piece of cheek skin, rostroventral to the eye, was removed from the left (denervated) and the right (intact) sides and processed for light microscopy or immunocytochemistry. Our results show: (1) there is progressive receptor cell death and tuberous organ degeneration following denervation; (2) basal cell proliferation increases steadily with time after denervation and tuberous organ degeneration; and (3) despite denervation, some proliferating basal cells differentiate into receptor cells, but these new receptor cells eventually die. These results suggest that innervation is essential for tuberous electroreceptor cell survival and that the rate at which basal cells proliferate is regulated by receptor cell health, locally released factors, or both. © 1994 Wiley‐Liss, Inc.