THE GROES AND GROEL HEAT-SHOCK GENE-PRODUCTS OF ESCHERICHIA-COLI ARE ESSENTIAL FOR BACTERIAL-GROWTH AT ALL TEMPERATURES
THE GROES AND GROEL HEAT-SHOCK GENE-PRODUCTS OF ESCHERICHIA-COLI ARE ESSENTIAL FOR BACTERIAL-GROWTH AT ALL TEMPERATURES
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DOI：
10.1128/jb.171.3.1379-1385.1989
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发表时间：
1989-03-01
影响因子：
3.2
通讯作者：
GEORGOPOULOS, C
中科院分区：
文献类型：
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作者：
FAYET, O;ZIEGELHOFFER, T;GEORGOPOULOS, C
已知大肠杆菌的groES和groEL基因的产物构成groE操纵子，是高温（42 ℃）生长所需的，并且是热激调节子的成员。使用遗传方法，我们检测了细菌在低温（17 - 30 ℃）下生长对这些基因产物的需要。为此，我们构建了各种groES groEL异源二倍体衍生菌株。通过极性插入使其中一个groE操纵子失活，噬菌体P1转导表明，至少一个groE基因是低温生长所必需的。进一步的P1转导实验表明，只有一个groE基因的异源二倍体菌株的生长在低温下所需的两个groE基因的产品，这表明在大肠杆菌中的groE蛋白的基本作用。大肠杆菌的生长和生理。
The products of the groES and groEL genes of Escherichia coli, constituting the groE operon, are known to be required for growth at high temperature (42.degree.C) and are members of the heat shock regulon. Using a genetic approach, we examined the requirement for these gene products for bacterial growth at low temperature (17 to 30.degree.C). To do this, we constructed various groES groEL heterodiploid derivative strains. By inactivating one of the groE operons by a polar insertion, it was shown by bacteriophage P1 transduction that at least one of the groE genes was essential for growth at low temperature. Further P1 transduction experiments with strains that were heterodiploid for only one of the groE genes demonstrated that both groE gene products were required for growth at low temperature, which suggested a fundamental role for the groE proteins in E. coli growth and physiology.