Blood BDNF concentrations reflect brain-tissue BDNF levels across species
Blood BDNF concentrations reflect brain-tissue BDNF levels across species
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DOI：
10.1017/s1461145710000738
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发表时间：
2011-04-01
影响因子：
4.8
通讯作者：
Aznar, Susana
中科院分区：
文献类型：
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作者：
Klein, Anders B.;Williamson, Rebecca;Aznar, Susana
脑源性神经营养因子（BDNF）参与突触可塑性、神经元分化和神经元存活。在神经精神疾病患者中观察到血清BDNF水平降低，突出了BDNF作为生物标志物的潜力，但到目前为止，还没有直接比较不同物种血液BDNF水平与脑BDNF水平的研究。我们研究了血液，血清，血浆和脑组织中的BDNF水平在三个不同的哺乳动物物种：大鼠，猪和小鼠，使用ELISA方法。作为对照，我们分析了条件性BDNF基因敲除小鼠及其野生型同窝仔的血液和脑组织。BDNF在大鼠的血液、血浆和脑组织中很容易检测到，但在小鼠的血液中却检测不到。在猪中，BDNF的全血水平不能用市售的ELISA试剂盒测量，但猪血浆BDNF水平（平均994+/-186 pg/ml）与先前报道的人类值相当。我们证明大鼠全血BDNF水平和海马BDNF水平之间（r（2）=0.44，p=0.025）以及猪血浆BDNF和海马BDNF之间（r（2）=0.41，p=0.025）呈正相关。此外，我们发现小鼠额叶皮质和海马BDNF水平之间存在显著的正相关性（r（2）=0.81，p=0.0139）。我们的数据支持血液和血浆BDNF水平的测量反映脑组织BDNF水平的观点。
Brain-derived neurotrophic factor (BDNF) is involved in synaptic plasticity, neuronal differentiation and survival of neurons. Observations of decreased serum BDNF levels in patients with neuropsychiatric disorders have highlighted the potential of BDNF as a biomarker, but so far there have been no studies directly comparing blood BDNF levels to brain BDNF levels in different species. We examined blood, serum, plasma and brain-tissue BDNF levels in three different mammalian species : rat, pig, and mouse, using an ELISA method. As a control, we included an analysis of blood and brain tissue from conditional BDNF knockout mice and their wild-type littermates. Whereas BDNF could readily be measured in rat blood, plasma and brain tissue, it was undetectable in mouse blood. In pigs, whole-blood levels of BDNF could not be measured with a commercially available ELISA kit, but pig plasma BDNF levels (mean 994+/-186 pg/ml) were comparable to previously reported values in humans. We demonstrated positive correlations between whole-blood BDNF levels and hippocampal BDNF levels in rats (r(2)=0.44, p=0.025) and between plasma BDNF and hippocampal BDNF in pigs (r(2)=0.41, p=0.025). Moreover, we found a significant positive correlation between frontal cortex and hippocampal BDNF levels in mice (r(2)=0.81, p=0.0139). Our data support the view that measures of blood and plasma BDNF levels reflect brain-tissue BDNF levels.