Effect of synthetic progestational agents on allograft rejection and circulating antibody production.
Effect of synthetic progestational agents on allograft rejection and circulating antibody production.
复制标题
合成孕激素对同种异体移植排斥和循环抗体产生的影响。
作者：
J. Hulka;K. Mohr;M. Lieberman
本研究评价了合成孕激素对免疫反应的影响。体重在3至4公斤之间的成年白兔被手术阉割。1周后给予5只兔类固醇，剂量为人体治疗剂量上限的5倍。每只经可的松治疗的动物腹腔注射4 mEq氯化钾溶液。对照组包括阉割和未阉割的动物，以及单独接受丙二醇或油的动物。在类固醇治疗至少1周后进行同种异体移植物交换。对照异体移植物无排斥反应。正常和阉割动物的平均排斥时间为9天。可的松治疗的动物耐受同种异体移植物至少3周。雌二醇和黄体酮、合成黄体酮、己酸17-羟基黄体酮和醋酸甲羟黄体酮对移植物存活无影响。“19 nor”组合成孕药的去甲thindrone和去甲nodrel化合物显著延长同种异体移植物的存活时间，平均为13天。使用F检验对阉割组和接受黄体酮、甲羟黄体酮和可的松治疗的组进行的一系列方差分析表明，这些化合物显著减少了循环抗体的产生。接受甲孕酮治疗的动物和接受可的松治疗的动物的反应没有差异。研究结果表明，移植排斥反应和抗体产生的机制非常不同，不同的类固醇对其抑制程度不同。应该做进一步的研究来确定这些孕激素对人类免疫反应的影响。
This study evaluates the effects of synthetic progestational agents on immune responses. Adult white rabbits weighing between 3 and 4 kg were surgically castrated. 5 rabbits were given a steroid 1 week later in a dose 5 times the upper limit of its human therapeutic dose. 4 mEq of potassium chloride solution were administered intraperitoneally each in cortisone-treated animals. Control groups included castrated and noncastrated animals, and those receiving propylene glycol or oil alone. Allograft exchanges were performed after at least 1 week of steroid administration. No control allograft was rejected. Normal and castrated animals had an average rejection time of 9 days. Cortisone-treated animals tolerated their allografts for at least 3 weeks. Graft survival was unaffected by estradiol and progesterone, synthetic progestins, 17-hydroxyprogesterone caproate and medroxyprogesterone acetate. Allograft survival was significantly prolonged to an average of 13 days by norethindrone and norethynodrel compounds of the "19 nor" group of synthetic progestational agents. A series of analyses of variance using the 'F' test between the castrated group and those receiving progesterone, medroxyprogesterone and cortisone indicated that these compounds significantly reduced circulating antibody production. There was no difference in the response of animals receiving medroxyprogesterone and those receiving cortisone. The findings show that the mechanisms of graft rejection and antibody production are sufficiently distinct to be inhibited by different steroids in different degrees. Further research should be done to determine the effects of these progestational agents on immune responses in humans.