Culture-Based and Culture-Independent Assessments of Endophytic Fungal Diversity in Aquatic Plants in Southwest China.
Culture-Based and Culture-Independent Assessments of Endophytic Fungal Diversity in Aquatic Plants in Southwest China.
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DOI：
10.3389/ffunb.2021.692549
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发表时间：
2021
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水生生态系统包含了巨大的植物和微生物多样性。然而，对水生植物内生菌的多样性知之甚少。本研究采用基于培养和非培养的高通量测序方法对中国西南地区水生植物内生真菌的多样性进行了研究。从30种水生植物的15,373个组织切片中分离到真菌1,689个，隶属于3门154属。内生真菌以子囊菌属的曲霉属、角点霉属、镰刀菌属、青霉属、茎点霉属和丛孢霉属的内生真菌最丰富。根、茎、叶的真菌分离率无显著差异。对来自三种最常见植物物种的二十个组织样品进一步进行非培养物依赖性元条形码测序。基于序列的分析显示，共有1，074个OTU属于6个真菌门和194个属。在这三种植物中，山毛榉的OTU数量最多。此外，两种方法共检测到66个属。非培养和非培养的方法均表明西南地区水生植物内生真菌多样性丰富。这项研究大大扩展了我们对水生植物真菌群落的了解。
Aquatic ecosystems contain tremendous plant and microbial diversity. However, little is known about endophyte diversity in aquatic plants. In this study, we investigated the diversity of endophytic fungi in aquatic plants in southwest China using both culture-based and culture-independent high-throughput sequencing methods. A total of 1,689 fungal isolates belonging to three phyla and 154 genera were obtained from 15,373 plant tissue segments of 30 aquatic plant species. The most abundant endophytic fungi were those in ascomycete genera Aspergillus, Ceratophoma, Fusarium, Penicillium, Phoma and Plectosporium. No difference in fungal isolation rates was observed among tissues from roots, stems, and leaves. Twenty tissue samples from three most common plant species were further subjected to culture-independent meta-barcode sequencing. The sequence-based analyses revealed a total of 1,074 OTUs belonging to six fungal phyla and 194 genera. Among the three plants, Batrachium bungei harbored the highest number of OTUs. Besides, a total of 66 genera were detected by two methods. Both the culture-dependent and independent methods revealed that aquatic plants in southwest China have abundant endophytic fungal diversity. This study significantly expands our knowledge of the fungal community of aquatic plants.