Polymorphism and chromosomal localization of the GI-form of human glutathione peroxidase (GPX2) on 14q24.1 by in situ hybridization.
Polymorphism and chromosomal localization of the GI-form of human glutathione peroxidase (GPX2) on 14q24.1 by in situ hybridization.
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通过原位杂交对 14q24.1 上的人谷胱甘肽过氧化物酶 (GPX2) GI 形式进行多态性和染色体定位。
DOI：
10.1006/geno.1996.0115
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
Pettenati,MJ
中科院分区：
文献类型：
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作者：
Chu,FF;RohandeSilva,HA;Esworthy,RS;Boteva,KK;Walters,CE;Roses,A;Rao,PN;Pettenati,MJ
我们分离出了人类gpx2基因的一个3.3 kb的DNA，包含两个外显子和一个2.6 kb的内含子。该基因编码谷胱甘肽过氧化物酶肠道同工酶GSHPx-GI。当将该DNA用作中期染色体荧光原位杂交探针时，在14q24.1处检测到一致的信号。无论是单链构象多态性还是测序分析，均发现内含子存在两个DNA多态性位点：5′端存在单个a /T改变，3′端存在TC重复序列微卫星。
We have isolated a 3.3-kb DNA containing the two exons and a 2.6-kb intron of the humanGPX2gene. This gene encodes the intestinal isoenzyme of glutathione peroxidase, GSHPx-GI. Consistent signals were detected at 14q24.1 when this DNA was used as a probe for fluorescencein situhybridization on metaphase chromosomes. Based on either single-stranded conformation polymorphism or sequencing analysis, two sites containing DNA polymorphism were found in the intron: the 5′ end had a single A/T alteration, and the 3′ end had a microsatellite of TC repeats.