Metabolites of the Aquatic Pollutant Diclofenac in Fish Bile
Metabolites of the Aquatic Pollutant Diclofenac in Fish Bile
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DOI：
10.1021/es903402c
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发表时间：
2010-10-01
影响因子：
11.4
通讯作者：
Kronberg, Leif
中科院分区：
文献类型：
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作者：
Kallio, Jenny-Maria;Lahti, Marja;Kronberg, Leif
研究了抗炎药物双氯芬酸(DCF)经腹腔染毒和经水染毒的吸收代谢规律。收集胆汁并鉴定形成的代谢物。鉴定的依据是飞行时间质量分析器的准确质量测定和离子陷阱质量分析器对前体离子碎片和碎片模式的研究。主要代谢产物为羟基化DCFs的酰基葡萄糖醛酸类化合物。此外，还发现了一种羟化DCF的乙醚葡萄糖醛酸根。此外，在胆汁中也检测到未代谢的DCF。虹鱼胆汁中的总生物浓缩系数(BCFTAL-DCF及其代谢物的胆汁)因个体而异，粗略估计在320至950之间。这些发现表明，生活在污水处理厂(WWTP)下游并长期接触该药物的鱼类可能会在胆汁中积累该药物及其代谢物。
The uptake and metabolism of anti-inflammatory drug diclofenac (DCF) was studied by exposing rainbow trout (Oncorhynchus mykiss) to DCF intraperitoneally, and via water at concentration of 1.7 mu g L-1. The bile was collected and the formed metabolites were identified. The identification was based on the exact mass determinations by a time-of-flight mass analyzer and on the studies of fragments and fragmentation patterns of precursor ions by an ion trap mass analyzer. The main metabolites found were acyl glucuronides of hydroxylated DCFs. In addition, one ether glucuronide of hydroxylated DCF was found. Also, unmetabolized DCF was detected in the bile. The total bioconcentration factors (BCFtotal-bile for DCF and its metabolites) in rainbow trout bile, varied between individuals and was roughly estimated to range from 320 to 950. These findings suggest that fish living downstream the wastewater treatment plants (WWTPs) and which are chronically exposed to the drug may accumulate the drug and its metabolites in the bile.