Rosetting of antibody-sensitized tumor cells with anti-immunoglobulin antibody-coated erythrocytes by a new method for detecting antigens on cells.
Rosetting of antibody-sensitized tumor cells with anti-immunoglobulin antibody-coated erythrocytes by a new method for detecting antigens on cells.
复制标题
通过检测细胞上抗原的新方法，用抗免疫球蛋白抗体包被的红细胞使抗体致敏的肿瘤细胞玫瑰花化。
作者：
G. Molinaro;K. Sato;R. Paque;S. Dray
用兔抗肿瘤相关细胞表面抗原抗体处理肿瘤细胞，并用包被兔免疫球蛋白特异性抗体的红细胞进行检测。致敏的肿瘤细胞与指示细胞形成花环。通过这种方法，我们证实了1号和10号肝癌细胞（来自菌株2豚鼠中由二乙基亚硝胺独立诱导的两个肿瘤）携带正常肝细胞不存在的抗原。我们还证实，1号和10号细胞系具有不同的肿瘤相关细胞表面抗原。这种方法比其他血清学方法更容易检测肿瘤细胞上的肿瘤相关细胞表面抗原。此外，该方法可作为检测和枚举携带表面抗原的细胞的一般方法。
Tumor cells were treated with rabbit antibody to tumor-associated cell surface antigens and tested with erythrocytes coated with antibody specific for the sensitizing rabbit immunoglobulin. The sensitized tumor cells formed rosettes with the indicator cells. By this method, we confirmed that line 1 and line 10 hepatoma cells (from two tumors independently induced by diethylnitrosamine in strain 2 guinea pigs) bear antigens not present on normal liver cells. We also confirmed that line 1 and line 10 cells bear antigenically different tumor-associated cell surface antigens. This method appears simpler than other serological methods for detecting tumor-associated cell surface antigens on tumor cells. Also, this method may be a general one for detecting and enumerating cells bearing surface antigens.