Initiation of SV40 DNA replication: mechanism and control.
Initiation of SV40 DNA replication: mechanism and control.
复制标题
DOI：
10.1101/sqb.1991.056.01.037
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
L. Erdile;Kathleen L. Collins;Alicia Russo;Pamela Simancek;Donald Small;Christopher B. Umbricht;David M. Virshup;Linzhao Cheng;S. Randall;D. H. Weinberg;I. Moarefi;E. Fanning;Thomas J. Kelly
中科院分区：
文献类型：
--
作者：
L. Erdile;Kathleen L. Collins;Alicia Russo;Pamela Simancek;Donald Small;Christopher B. Umbricht;David M. Virshup;Linzhao Cheng;S. Randall;D. H. Weinberg;I. Moarefi;E. Fanning;Thomas J. Kelly
细胞基因组的复制是细胞周期中的关键事件。DNA复制开始的时间受到严格控制，并且存在确保染色体的每个片段复制一次且仅复制一次的机制。为了更好地理解这些控制机制，识别和表征参与DNA复制的蛋白质并研究它们的功能相互作用至关重要。由于真核染色体的大小和复杂性使得细胞DNA复制的详细分析变得困难，我们选择使用SV 40作为模型系统。该病毒模型的实用性源于这样的事实，即SV 40 DNA的复制仅需要单个病毒蛋白（SV 40大T抗原）的参与，因此DNA合成所需的大多数酶功能由细胞蛋白提供。此外，控制病毒DNA复制的机制可能与调节细胞DNA复制的机制相似（有关综述，参见Challberg和Kelly（1989）; Stillman（1989）;由于病毒和细胞的DNA复制可能在起始水平受到调节，因此在本文中，本文综述了近年来我们在引发反应的生化机理方面的工作。我们描述了起始蛋白之间的功能相互作用，并总结了已知的控制起始效率的因素。最后，我们提出了一些想法，这些数据可能的相关性，在细胞周期中的DNA复制的调节。
The replication of the cellular genome is a critical event in the cell cycle. The time of onset of DNA replication is tightly controlled, and mechanisms exist to ensure that each segment of the chromosome is duplicated once and only once. To better understand these control mechanisms, it is essential to identify and characterize the proteins involved in DNA replication and to study their functional interactions. Since the size and complexity of the eukaryotic chromosome make detailed analysis of cellular DNA replication difficult, we have chosen to use SV40 as a model system. The utility of this viral model derives from the fact that replication of SV40 DNA requires the participation of only a single viral protein, the SV40 large T antigen, so that most of the enzymatic functions necessary for DNA synthesis are provided by cellular proteins. In addition, the mechanisms involved in the control of viral DNA replication may be similar to those regulating the replication of cellular DNA (for review, see Challberg and Kelly 1989; Stillman 1989; Hurwitz et al. 1990).Since DNA replication, both viral and cellular, is likely to be regulated at the level of initiation, in this paper, we summarize our recent work on the biochemical mechanism of the initiation reaction. We describe the functional interactions among the initiation proteins and summarize what is known about the factors that control the efficiency of initiation. Finally, we present some ideas on the possible relevance of these data to the regulation of DNA replication during the cell cycle.