Rate of nuclear ribonucleic acid turnover in sea urchin embryos.
Rate of nuclear ribonucleic acid turnover in sea urchin embryos.
复制标题
海胆胚胎中核糖核酸周转率。
DOI：
10.1016/0022-2836(69)90472-0
复制
发表时间：
1969
影响因子：
5.6
通讯作者：
F. Wilt
中科院分区：
文献类型：
--
作者：
S. Kijima;F. Wilt
在海胆胚胎发育过程中，[3 H]鸟苷掺入到三磷酸鸟苷和水解RNA的2′-3′磷酸鸟苷中。计算总RNA中的鸟苷一磷酸的量，其成为放射性的（10分钟的时间），并且周转值的范围从0.7%的中期卵裂阶段到约1.3%的pluteus幼虫。随着发育的进行，每个基因组的RNA合成速率实际上是下降的，因为细胞复制的速率超过了每个胚胎RNA合成速率的增加。标记RNA的动力学表明，初始掺入率没有保持，并且很可能大量新产生的RNA是不稳定的。放射自显影显示，在原肠胚形成之前，大部分新产生的RNA至少在两小时内仍局限于细胞核。有人提出，不稳定的核RNA是定量的早期发育过程中合成的RNA的重要组成部分。
RNA synthesis in the developing sea urchin embryo was characterized by following the incorporation of [3H]guanosine into guanosine triphosphate and the 2′-3′ guanosine monophosphate of hydrolyzed RNA. The amount of guanosine monophosphate in the total RNA which became radioactive was calculated (for a 10-min period), and the turnover values range from 0.7% for a mid-cleavage stage to about 1.3% for the pluteus larva. Therate of RNA synthesis per genome actually decreasedas development proceeded, for the rate of cell replication exceeded the increase in RNA synthesis per embryo. The kinetics of labeling of RNA showed that initial incorporation rates were not maintained, and it is probable that a substantial amount of newly made RNA is unstable. Autoradiograms showed that prior to gastrulation much of the newly made RNA remained restricted to the nucleus for at least two hours. It is proposed that labile nuclear RNA is quantitatively an important part of the RNA synthesized during early development.