Effect of a low temperature tolerant lactic acid bacteria inoculant on the fermentation quality and bacterial community of oat round bale silage
Effect of a low temperature tolerant lactic acid bacteria inoculant on the fermentation quality and bacterial community of oat round bale silage
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DOI：
10.1016/j.anifeedsci.2020.114669
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发表时间：
2020-11-01
影响因子：
3.2
通讯作者：
Cai, Yimin
中科院分区：
文献类型：
--
作者：
Chen, Liangyin;Bai, Shiqie;Cai, Yimin
本试验旨在研究一种耐低温乳酸菌（LAB）接种剂对燕麦青贮发酵特性和细菌群落的影响。燕麦在开花期收获，枯萎后干物质含量约为270克/公斤鲜重。用枯萎和切碎的燕麦制作小型圆捆青贮饲料。在不添加（对照）或添加耐低温乳酸菌（包括植物乳杆菌BP18、戊糖Pediococcus HS1和l）的情况下制备青贮饲料，乳酸菌接种的青贮饲料中水溶性碳水化合物（WSC）、粗蛋白质（CP）、中性洗涤纤维（aNDF）和酸性洗涤纤维（ADF）含量较高。接种乳酸菌提高了H位青贮的乳酸和乙酸含量（P < 0.05），降低了H位青贮的终pH （P < 0.05）。而接种乳酸菌降低了L位青贮的细菌操作分类单位（OTUs）、丰富度指数（Chao 1）和多样性指数（Shannon） （P < 0.05）。青贮样品中优势菌属为乳杆菌属（Lactobacillus）、白菌属（Leuconostoc）和梭状芽孢杆菌属（Clostridium），总相对丰度为71%，接近93%。乳酸菌接种增加了青贮中理想乳酸菌的丰度，抑制了不理想梭状芽胞杆菌的生长。因此，在青贮阶段接种耐低温乳酸菌可以促进有利发酵，重建细菌群落，更好地保存高水分燕麦青贮营养物质。
The objective of this study was to investigate the effect of a low temperature tolerant lactic acid bacteria (LAB) inoculant on the fermentation characteristics and bacterial community of oat silage. Oats were harvested at the flowering stage and wilted to dry matter content of approximately 270 g/kg fresh weight. Wilted and chopped oats were used to make mini round bale silages. Silages were prepared without (control) or with a low temperature tolerant LAB inoculant including Lactobacillus plantarum BP18, Pediococcus pentosaceus HS1 and L. buchneri LP22 and then separately stored at different geographic locations (L, Chengdu Plain; H, Qinghai Tibetan Plateau) for 120 days. High contents of water soluble carbohydrate (WSC), crude protein (CP), neutral detergent fiber (aNDF) and acid detergent fiber (ADF) were found in LAB inoculated silage. Inoculation with LAB increased (P < 0.05) lactic acid and acetic acid contents, and decreased (P < 0.05) the final pH of silage stored at the H location. However, LAB inoculation reduced (P < 0.05) the bacterial operational taxonomic units (OTUs), richness index (Chao 1) and diversity index (Shannon) of silage stored at the L location. The dominant genera from silage samples were Lactobacillus, Leuconostoc and Clostridium, with a total relative abundance of 71 similar to 93 %. LAB inoculation increased the abundance of desirable Lactobacillus and inhibited the growth of undesirable Clostridium in silage. Therefore, inoculation of low temperature tolerant LAB at ensiling could stimulate favorable fermentation and reconstruct the bacterial community for better preservation of high moist oat silage nutrients.