Genetic instability of clathrin-deficient strains of Saccharomyces cerevisiae.
Genetic instability of clathrin-deficient strains of Saccharomyces cerevisiae.
复制标题
网格蛋白缺陷型酿酒酵母菌株的遗传不稳定性。
DOI：
10.1093/genetics/124.1.27
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发表时间：
1990
期刊：
影响因子：
3.3
通讯作者：
Jones,EW
中科院分区：
文献类型：
--
作者：
Lemmon,SK;Freund,C;Conley,K;Jones,EW
携带网格蛋白重链基因（CHC 1）突变的酿酒酵母菌株在遗传上不稳定，并产生异质细胞群。这种不稳定性的表现包括基因组拷贝数的增加以及补偿性遗传变化，这些变化允许更好地生长网格蛋白缺陷细胞接管种群。基因组拷贝数的增加似乎是由于倍性的变化以及正常细胞核数目的改变。遗传背景影响在不同Chc-菌株中观察到基因组含量增加的细胞的频率。我们无法区分遗传背景是否影响异常核分裂事件发生的速率，或者核和/或基因组含量增加的细胞的生长优势。然而，chc 1-delta细胞的存活并不需要基因组拷贝数的增加。通过利用整合的2 μ质粒序列使远端染色体区段不稳定并允许基因排序，将网格蛋白重链基因定位在染色体VII左臂上KEX 1远端1-2 cM处。
Saccharomyces cerevisiae strains carrying a mutation in the clathrin heavy chain gene (CHC1) are genetically unstable and give rise to heterogeneous populations of cells. Manifestations of the instability include increases in genome copy number as well as compensatory genetic changes that allow better growing clathrin-deficient cells to take over the population. Increases in genome copy number appear to result from changes in ploidy as well as alterations in normal nuclear number. Genetic background influences the frequency at which cells with increased genome content are observed in different Chc- strains. We cannot distinguish whether genetic background affects the rate at which aberrant nuclear division events occur or a growth advantage of cells with increased nuclear and/or genome content. However, survival of chc1-delta cells does not require an increase in genome copy number. The clathrin heavy chain gene was mapped 1-2 cM distal to KEX1 on the left arm of chromosome VII by making use of integrated 2 mu plasmid sequences to destabilize distal chromosome segments and allow ordering of the genes.