Administration of recombinant human interleukin-7 alters the frequency and number of myeloid progenitor cells in the bone marrow and spleen of mice.
Administration of recombinant human interleukin-7 alters the frequency and number of myeloid progenitor cells in the bone marrow and spleen of mice.
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DOI：
10.1182/blood.v79.5.1121.1121
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发表时间：
1992-03
期刊：
影响因子：
20.3
通讯作者：
G. Damia;K. Komschlies;C. Faltynek;F. Ruscetti;R. Wiltrout
中科院分区：
文献类型：
--
作者：
G. Damia;K. Komschlies;C. Faltynek;F. Ruscetti;R. Wiltrout
每天两次给予小鼠大于或等于5微克的白细胞介素-7（IL-7），持续4至7天，使脾脏和外周血白细胞总数增加2倍至5倍，但并未明显增加骨髓（BM）细胞构成。这种IL-7给药方案还导致可从BM培养的单系集落形成单位培养物（CFU-c）和多系CFU-粒细胞、红细胞、单核细胞、巨核细胞集落的频率和总数减少90%以上，但可从脾培养的这些祖细胞的数量增加5倍至15倍。所有这些影响都是可逆的，祖细胞和白色血细胞数量在第6天恢复到接近正常。从IL-7处理的小鼠的BM获得的细胞的形态学分析显示淋巴样细胞的增加。表面表型分析表明，IL-7诱导的淋巴细胞增加主要归因于未成熟B细胞（B220+，sIg-）的增加，而表达骨髓单核细胞标志物8 C5和MAC-1的细胞减少了2 - 3倍。进一步的研究表明，与单独使用环磷酰胺（Cy）或5-氟尿嘧啶（5-FU）治疗的小鼠相比，对注射环磷酰胺（Cy）或5-FU而导致白细胞减少的小鼠给予IL-7，其外周血淋巴细胞的恢复和/或过冲表现得更快。这些结果表明，IL-7可以差异调节骨髓和脾脏中的骨髓生成，同时刺激淋巴细胞生成。
The administration of greater than or equal to 5 micrograms interleukin-7 (IL-7) twice a day to mice for 4 to 7 days increased by twofold to fivefold the total number of splenic and peripheral blood leukocytes, but did not appreciably increase bone marrow (BM) cellularity. This regimen of IL-7 administration also resulted in a greater than 90% reduction in the frequency and total number of single lineage colony-forming unit-culture (CFU-c) and multilineage CFU-granulocyte, erythroid, monocyte, megakaryocyte colonies that could be cultured from the BM, but a fivefold to 15-fold increase in the number of these progenitors that could be cultured from the spleen. All of these effects were reversible with progenitor and white blood cell numbers returning to near normal by day 6. Morphologic analysis of cells obtained from the BM of IL-7-treated mice showed an increase in lymphoid cells. Surface phenotype analysis showed that most of this IL-7-induced increase in lymphocytes was attributable to an increase in immature B cells (B220+, sIg-), while cells expressing the myelomonocytic markers 8C5 and MAC-1 decreased by twofold to threefold. Further studies showed that the administration of IL-7 to mice that had been rendered leukopenic by the injection of cyclophosphamide (Cy) or 5-fluorouracil (5FU) exhibited a more rapid recovery and/or overshoot in their peripheral blood lymphocytes when compared with mice treated with Cy or 5FU alone. These results show that IL-7 can differentially regulate myelopoiesis in the BM and spleen, while stimulating lymphopoiesis.