Arabidopsis YL1/BPG2 Is Involved in Seedling Shoot Response to Salt Stress through ABI4.
Arabidopsis YL1/BPG2 Is Involved in Seedling Shoot Response to Salt Stress through ABI4.
复制标题
拟南芥 YL1/BPG2 通过 ABI4 参与幼苗对盐胁迫的响应
DOI：
10.1038/srep30163
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发表时间：
2016-07-22
影响因子：
4.6
通讯作者：
Zheng CC
中科院分区：
文献类型：
--
作者：
Li PC;Huang JG;Yu SW;Li YY;Sun P;Wu CA;Zheng CC
叶绿体定位蛋白在植物盐胁迫响应中发挥作用，但其作用机制尚不清楚。在本研究中，我们筛选了一个黄叶突变体，yl 1 -1，其芽表现出对盐胁迫敏感。我们将YL 1定位到AT 3G 57180，其编码YqeH型GT3。YL 1是叶绿体基质蛋白，在高盐条件下含量显著降低。在高盐胁迫下，yl 1 -1和yl 1 -2幼苗的Na+积累量显著高于野生型。对植物盐胁迫响应相关因子的表达分析表明，在正常生长条件下，突变体中ABI 4的表达量明显高于野生型，而HKT 1的表达量明显低于野生型。此外，盐对ABI 4和HKT 1的影响在突变体芽中明显减弱，表明YL 1功能的丧失损害了ABI 4和HKT 1的表达。与yl 1 -2单突变体相比，yl 1 -2 abi 4双突变体的幼苗表现出更强的耐盐性和更少的Na+积累量，表明yl 1 -2幼苗的盐敏感表型可以通过ABI 4功能的丧失来挽救。这些结果表明，YL 1通过ABI 4参与了幼苗盐胁迫反应。
The chloroplast-localized proteins play roles in plant salt stress response, but their mechanisms remain largely unknown. In this study, we screened a yellow leaf mutant, yl1-1, whose shoots exhibited hypersensitivity to salt stress. We mapped YL1 to AT3G57180, which encodes a YqeH-type GTPase. YL1, as a chloroplast stroma-localized protein, could be markedly reduced by high salinity. Upon exposure to high salinity, seedling shoots of yl1-1 and yl1-2 accumulated significantly higher levels of Na+ than wild type. Expression analysis of factors involved in plant salt stress response showed that the expression of ABI4 was increased and HKT1 was evidently suppressed in mutant shoots compared with the wild type under normal growth conditions. Moreover, salinity effects on ABI4 and HKT1 were clearly weakened in the mutant shoots, suggesting that the loss of YL1 function impairs ABI4 and HKT1 expression. Notably, the shoots of yl1-2 abi4 double mutant exhibited stronger resistance to salt stress and accumulated less Na+ levels after salt treatment compared with the yl1-2 single mutant, suggesting the salt-sensitive phenotype of yl1-2 seedlings could be rescued via loss of ABI4 function. These results reveal that YL1 is involved in the salt stress response of seedling shoots through ABI4.