Simple enzyme immunoassay for the measurement of immunoreactive chromogranin A in human plasma, urine and saliva
Simple enzyme immunoassay for the measurement of immunoreactive chromogranin A in human plasma, urine and saliva
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用于测量人血浆、尿液和唾液中免疫反应性嗜铬粒蛋白 A 的简单酶免疫分析
DOI：
10.2220/biomedres.19.407
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发表时间：
1998
影响因子：
1.2
通讯作者：
N. Yanaihara
中科院分区：
文献类型：
--
作者：
S. Nagasawa;Yasuko Nishikawa;Jun Li;Y. Futai;T. Kanno;K. Iguchi;T. Mochizuki;M. Hoshino;C. Yanaihara;N. Yanaihara
以人工合成的人嗜铬粒蛋白A（CgA）（344-374）为抗原，建立了一种简便、灵敏的测定人CgA样免疫反应性（IR）的酶免疫分析法（EIA）。在本EIA系统的开发中，特意设计合成的N”-生物素化甘氨酰甘氨酰-人CgA（344-374）被证明是有用的生物素化衍生物。典型的标准曲线范围为13.7 fmol/mL至10 pmol/mL的CgA样IR。在本EIA中，人血浆、尿液和唾液的稀释曲线与合成人CgA的标准曲线一致（344-374）。人血浆CgA测定的批内变异系数为2.6%-8.5%，表明可对人血浆进行可靠测定。正常人空腹血浆、晨尿和唾液中CgA-like IR水平分别为0.34 ± 0.08 pmol/mL（平均± SD，n = 10）、0.181 ± 0.05 pmol/mL（n=8）和0.1135 ± 0.05 pmol /mL（n = 8）。
A simple and sensitive enzyme immunoassay (EIA) was developed for determining human chromogranin A (CgA) -like immunoreactivity (IR) with use of synthetic human CgA (344-374) as antigen. In the development of the present EIA system, purposely designed N“-biotinylated glycylglycyl-human CgA (344-374), which was synthesized, was proved to be a useful biotinylated derivative. Typical standard curves ranged from 13.7 fmol/mL to 10 pmol/mL of CgA-like IR. In the present EIA, the curves for dilution of human plasma, urine, and saliva paralleled the standard curve for synthetic human CgA (344-374). The intra-assay coefficient of variation for measurement of human plasma CgA was from 2.6% to 8.5%, indicating that reliable determinations can be performed for human plasma. The levels of CgA-like IR were 0.34i0.08 pmol/mL (meaniSD, n: 10) in fasting normal plasma, 0.181: 0.05 pmol/mL (n=8) in urine, and 0.1 1350.05 pmol /mL (n:8) in saliva in the morning are