Analysis of the Plasmodium falciparum proteome by high-accuracy mass spectrometry
Analysis of the Plasmodium falciparum proteome by high-accuracy mass spectrometry
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DOI：
10.1038/nature01111
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发表时间：
2002-10-03
期刊：
影响因子：
64.8
通讯作者：
Mann, M
中科院分区：
文献类型：
--
作者：
Lasonder, E;Ishihama, Y;Mann, M
生物体的注释基因组定义了它们可能的基因产物的“蓝图”。后基因组分析试图确认和修改注释，并强加一种生物体对遗传信息的空间、时间和发育使用的感觉。在这里，我们描述了一个大规模的，高精度（平均偏差小于0.02 Da，在1,000 Da）质谱蛋白质组分析（1-3）的选择阶段的人类疟疾寄生虫恶性疟原虫。分析揭示了1，289种蛋白质，其中714种蛋白质在无性血液阶段被鉴定，931种在配子体中，645种在配子中。最后两组提供了深入了解寄生虫的性阶段的生物学，包括保守的，阶段特异性的，分泌的和膜相关的蛋白。这些蛋白质的一个子集含有表明在细胞-细胞相互作用中的作用的结构域，因此可以作为疟疾疫苗制剂的潜在组分进行评估。我们还报告了一组肽与寄生虫基因组中的显着匹配，但不是在通过计算方法预测的蛋白质组。
The annotated genomes of organisms define a 'blueprint' of their possible gene products. Post-genome analyses attempt to confirm and modify the annotation and impose a sense of the spatial, temporal and developmental usage of genetic information by the organism. Here we describe a large-scale, high-accuracy (average deviation less than 0.02 Da at 1,000 Da) mass spectrometric proteome analysis(1-3) of selected stages of the human malaria parasite Plasmodium falciparum. The analysis revealed 1,289 proteins of which 714 proteins were identified in asexual blood stages, 931 in gametocytes and 645 in gametes. The last two groups provide insights into the biology of the sexual stages of the parasite, and include conserved, stage-specific, secreted and membrane-associated proteins. A subset of these proteins contain domains that indicate a role in cell-cell interactions, and therefore can be evaluated as potential components of a malaria vaccine formulation. We also report a set of peptides with significant matches in the parasite genome but not in the protein set predicted by computational methods.