Investigation of the Separation Efficiency of Tube Radial Distribution Chromatography with Stationary Outer Phase Using the van Deemter Equation
Investigation of the Separation Efficiency of Tube Radial Distribution Chromatography with Stationary Outer Phase Using the van Deemter Equation
复制标题
使用范第姆特方程研究固定外相管径向分布色谱的分离效率
DOI：
10.1007/s10337-019-03837-3
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发表时间：
2020
期刊：
影响因子：
1.7
通讯作者：
Kenichi Yamashita & Masaharu Murata
中科院分区：
文献类型：
--
作者：
Chihiro Matsushita;Kazuhiko Tsukagoshi;Katsumi Tsuchiya;Kenichi Yamashita & Masaharu Murata
径向分布色谱（TRDC）的外相是色谱分离中的固定相。使用TRDC在不同流速下，以熔融石英毛细管（内径，75 μm;长度，120 cm;有效长度，100 cm）为分离柱，以水/乙腈/乙酸乙酯（3：8：4，v/v/v）为洗脱液，检查模型分析物1-萘酚、1-萘磺酸、2，6-萘二磺酸和1，3，6-萘三磺酸的色谱峰信息。从数据中，平均线速度和相当于一个理论板的分析物的高度值之间的关系进行了研究，和TRDC，包括内相（移动的相）和外相（固定相）在一个开放的管状毛细管的分离效率，使用货车Deemter方程进行了检查。
The outer phase in tube radial distribution chromatography (TRDC) operated as a stationary phase in chromatographic separation. Chromatographic peak information from model analytes 1-naphthol, 1-naphthalenesulfonic acid, 2,6-naphthalenedisulfonic acid, and 1,3,6-naphthalenetrisulfonic acid was examined using TRDC at various flow rates, using a fused-silica capillary tube (inner diameter, 75 μm; length, 120 cm; effective length, 100 cm) as the separation column and water/acetonitrile/ethyl acetate (3:8:4, v/v/v) as eluent. From the data, the relationships between average linear velocities and values of height equivalent to one theoretical plate of the analytes were investigated, and the separation efficiency by TRDC, comprising an inner phase (mobile phase) and outer phase (stationary phase) in an open-tubular capillary tube, was examined using the van Deemter equation.