IlNRAMP5 is required for cadmium accumulation and the growth in Iris lactea under cadmium exposures.
IlNRAMP5 is required for cadmium accumulation and the growth in Iris lactea under cadmium exposures.
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DOI：
10.1016/j.ijbiomac.2023.127103
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发表时间：
2023-09
影响因子：
8.2
通讯作者：
Cui Li;Jia Zhang;Qidong Li;Zhimin Chen;Xincun Hou;Chunqiao Zhao;Qi Guo
中科院分区：
文献类型：
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作者：
Cui Li;Jia Zhang;Qidong Li;Zhimin Chen;Xincun Hou;Chunqiao Zhao;Qi Guo
鸢尾具有较强的Cd吸收和积累能力，具有修复Cd污染土壤的潜力。然而，其Cd摄取途径的分子机制尚不清楚。在这里，我们报道了NRAMP（天然耐药相关巨噬细胞蛋白）家族的一个成员IlNRAMP5，它参与了Cd/Mn摄取和细胞生长。IlNRAMP5定位于质膜上，受Cd诱导，在根皮质而非中央脉管系统中表达，在叶维管束和叶肉细胞中表达。在酵母中的异源表达表明，atilnramp5可以运输Cd和Mn，但不能运输Fe。敲低ilnramp5会导致Cd吸收显著减少，进一步减少Cd的积累。此外，沉默ilnramp5会通过降低Mn吸收和Mn分配来破坏Mn稳态，并显著抑制Cd条件下的光合作用。总的来说，研究结果表明IlNRAMP5通过介导Cd吸收在Cd积累中发挥了多种作用，并通过调节Mn稳态来维持生长。Cd暴露下的乳酸。这将为进一步了解镉在植物体内的修复效率提供机制依据。
Iris lacteais potentially applied for remediating Cd-contaminated soils due to the strong ability of Cd uptake and accumulation. However, its molecular mechanism underlying Cd uptake pathway remains unknown. Here, we report a member of NRAMP (Natural Resistance-Associated Macrophage Protein) family, IlNRAMP5, is involved in Cd/Mn uptake and the growth inI. lactearesponse to Cd. IlNRAMP5 was localized onto the plasma membrane, and was induced by Cd. It was expressed in the root cortex rather than the central vasculature, and in leaf vascular bundle and mesophyll cells. Heterologous expression in yeast showed thatIlNRAMP5could transport Cd and Mn, but not Fe. Knockdown ofIlNRAMP5triggered a significant reduction in Cd uptake, further diminishing the accumulation of Cd. In addition, silencingIlNRAMP5disrupted Mn homeostasis by lowering Mn uptake and Mn allocation, accompanied by remarkably inhibiting photosynthesis under Cd conditions. Overall, the findings suggest that IlNRAMP5 plays versatile roles in Cd accumulation by mediating Cd uptake, and contributes to maintain the growth via modulating Mn homeostasis inI. lacteaunder Cd exposures. This would provide a mechanistic understanding Cd phytoremediation efficiency inplanta.