Detection of Chlamydophila felis and feline herpesvirus by multiplex real-time PCR analysis
Detection of Chlamydophila felis and feline herpesvirus by multiplex real-time PCR analysis
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DOI：
10.1128/jcm.41.6.2734-2736.2003
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发表时间：
2003-06-01
影响因子：
9.4
通讯作者：
Harbour, D
中科院分区：
文献类型：
--
作者：
Helps, C;Reeves, N;Harbour, D
猫嗜衣原体和猫疱疹病毒（FHV）是猫呼吸道和眼部疾病的常见病原体。开发了一种实时多重PCR检测方法，可在单管中检测这些微生物以及猫28S核糖体DNA。检测538份眼拭子标本，FHV阳性123份，C阳性97份。16例两种病原体均呈阳性。
Chlamydophila felis and feline herpesvirus (FHV) are pathogens commonly associated with feline respiratory and ocular disease. A real-time multiplex PCR assay was developed to allow detection of these organisms, together with feline 28S ribosomal DNA, in a single tube. Of 538 ocular swab samples tested, 123 were positive for FHV, 97 were positive for C. felis, and 16 were positive for both pathogens.